Incomplete dsRNA genomes in purified infectious pancreatic necrosis virus

Virology
S KordybanP Dobos

Abstract

The dsRNA containing birnavirus, infectious pancreatic necrosis virus, possesses a virion-associated RNA-dependent RNA polymerase which acts both as primer and as polymerase during in vitro RNA synthesis (P. Dobos, 1995, Virology 208, 19-25). Using [alpha 32P]GTP, we have radiolabeled virion RNA in vitro and found that after deproteinization most of the labeled product comigrated in agarose gels with the 3-kbp viral genome, while the remainder migrated faster than the dsRNA and as a heterogeneous smear. Agarose gel electrophoresis (AGE) of denatured labeled virion RNA showed a radioactive smear ranging from approximately 100 nucleotides to up to 3000 nucleotide the size of genome length single stranded RNA. Hybridization experiments using strand-specific and end-specific obligonucleotides on Northern blots revealed that the radioactivity which migrates with the dsRNA during AGE represents small, 5' end plus RNA molecules of 100-500 nucleotides. The radioactivity in the faster migrating smear denotes incomplete dsRNAs where full-length, unlabeled minus strands are base-paired with labeled plus strands that are 3' truncated to different extents. This was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) using ...Continue Reading

References

Oct 1, 1991·The Journal of General Virology·J G CalvertP Dobos
Aug 25, 1973·Journal of Molecular Biology·J S SemancikJ L Van Etten
Mar 1, 1982·The Journal of General Virology·P P MertensP Dobos
Feb 1, 1980·Journal of Virology·J L Van EttenA K Vidaver
Jun 1, 1993·Journal of Virology·M W ChoE Ehrenfeld

❮ Previous
Next ❯

Citations

May 27, 2009·Virus Research·Marcelo Cortez-San MartínAna María Sandino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.