Inconsistencies in determining Bacillus thuringiensis toxin binding sites relationship by comparing competition assays with ligand blotting

Biochemical and Biophysical Research Communications
M K Lee, D H Dean

Abstract

Receptor binding properties of Cry1Aa, Cry1Ab, and Cry1Ac Bacillus thuringiensis toxins to Lymantria dispar brush border membrane vesicles (BBMV) were investigated by competition assays and BBMV ligand blotting. Homologous competition binding assays demonstrated that all Cry IA toxins bound to L. dispar BBMV with high binding affinities. Heterologous competition assays suggested that all three toxins share the same binding sites. However, our ligand blotting experiments were not consistent with the heterologous competition assays. We identified a 120 kDa peptide as a Cry1Ac binding protein and a 210 kDa peptide as a Cry1Aa and Cry1Ab binding protein. These results indicated that determining the relationships between toxin binding sites by either competition assays or ligand blotting alone may not be conclusive.

Citations

Jan 3, 2001·Applied and Environmental Microbiology·J L Jurat-Fuentes, M J Adang
Jul 8, 2005·Applied and Environmental Microbiology·Shogo AtsumiRyoichi Sato
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·F RajamohanD H Dean
May 5, 2004·The Journal of Biological Chemistry·Gang HuaMichael J Adang
Mar 3, 2021·Reports of Biochemistry & Molecular Biology·Sadegh RezaeiDariush Norouzian
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·E SchnepfD H Dean

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