PMID: 3748051Sep 1, 1986Paper

Incorporated bromodeoxyuridine enhances the sister-chromatid exchange and chromosomal aberration frequencies in an EMS-sensitive Chinese hamster cell line

Mutation Research
A V CarranoL H Thompson

Abstract

The mutant Chinese hamster cell line, EM9, is characterized by a high baseline sister-chromatid exchange (SCE) frequency, increased sensitivity to cell killing, and a defect in DNA strand-break repair. The molecular basis for this pleotrophic phenotype is not known. We examined, at the chromosomal level, the increased sensitivity of this mutant to incorporated BrdUrd. By varying the amount of BrdUrd in template DNA and measuring the frequency of SCEs and chromosomal aberrations, we demonstrated the enhanced sensitivity of EM9 to BrdUrd present in the template strand of DNA. Our results show that a 6-fold increase in SCEs occurs due to DNA replication over a BrdUrd-substituted template relative to a dThd-substituted template. With regard to aberration production in EM9, there is a significant enhancement of aberrations and a specific bias toward damage for the chromatid with Brdurd in the template strand. While these cells share some phenotypic properties with cells from patients with Bloom's syndrome, the genotypic similarities have not yet been established.

References

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Feb 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R Hand, J German
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Citations

Sep 2, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L H Thompson
Mar 1, 1994·Mutation Research·S LjungquistM Sandström
Mar 14, 2000·Mutation Research·L H Thompson, M G West
Sep 12, 2003·DNA Repair·Keith W Caldecott
May 4, 2005·Nucleic Acids Research·Reto Brem, Janet Hall
Mar 23, 2002·Molecular and Cellular Biology·Richard M TaylorKeith W Caldecott
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·N LiuL H Thompson
Nov 23, 2006·DNA Repair·Zhanna K NazarkinaJ Pablo Radicella
Jun 13, 2006·Mutation Research·Elisabeth LarsenArne Klungland
Jul 2, 2014·The Journal of Cell Biology·Michael EnsmingerMarkus Lӧbrich
Oct 20, 2007·Environmental and Molecular Mutagenesis·David M Wilson
Jun 5, 2019·PloS One·Ewa Wójcik, Małgorzata Szostek
Jul 22, 2019·DNA Repair·Keith W Caldecott
Dec 13, 2006·Mutation Research·David M Wilson, Larry H Thompson

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