Incorporating Bacteria as a Living Component in Supramolecular Self-Assembled Monolayers through Dynamic Nanoscale Interactions

ACS Nano
Shrikrishnan SankaranPascal Jonkheijm

Abstract

Supramolecular assemblies, formed through noncovalent interactions, has become particularly attractive to develop dynamic and responsive architectures to address living systems at the nanoscale. Cucurbit[8]uril (CB[8]), a pumpkin shaped macrocylic host molecule, has been successfully used to construct various self-assembled architectures for biomedical applications since it can simultaneously bind two aromatic guest molecules within its cavity. Such architectures can also be designed to respond to external stimuli. Integrating living organisms as an active component into such supramolecular architectures would add a new dimension to the capabilities of such systems. To achieve this, we have incorporated supramolecular functionality at the bacterial surface by genetically modifying a transmembrane protein to display a CB[8]-binding motif as part of a cystine-stabilized miniprotein. We were able to confirm that this supramolecular motif on the bacterial surface specifically binds CB[8] and forms multiple intercellular ternary complexes leading to aggregation of the bacterial solution. We performed various aggregation experiments to understand how CB[8] interacts with this bacterial strain and also demonstrate that it can be chemi...Continue Reading

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Citations

Nov 4, 2015·Chemistry : a European Journal·Ralph P G BosmansLuc Brunsveld
Aug 14, 2015·Bioconjugate Chemistry·Shrikrishnan SankaranPascal Jonkheijm
May 14, 2016·Chemical Communications : Chem Comm·Emanuela CavatortaPascal Jonkheijm
Jun 30, 2016·Angewandte Chemie·Ralph P G BosmansLuc Brunsveld
Jul 20, 2016·Chemistry : a European Journal·Tushar SatavPascal Jonkheijm
Dec 17, 2016·Physical Chemistry Chemical Physics : PCCP·Silvia SonziniOren A Scherman
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Jan 21, 2017·Angewandte Chemie·Theo PeschkeChristof M Niemeyer
May 19, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Shrikrishnan SankaranPascal Jonkheijm
Mar 24, 2018·RSC Advances·Emanuela CavatortaPascal Jonkheijm
Oct 10, 2019·Advanced Healthcare Materials·Oya Ilke SentürkSeraphine V Wegner
Nov 19, 2019·Chemical Communications : Chem Comm·Ranjan SasmalSarit S Agasti
Jan 27, 2018·Chemical Communications : Chem Comm·D WasserbergP Jonkheijm
May 28, 2021·Journal of Biomolecular Structure & Dynamics·Osmair Vital de OliveiraNatália Ellen Castilho de Almeida
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Apr 7, 2020·Journal of the American Chemical Society·Zehuan HuangOren A Scherman
Apr 26, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Hong DongDimitra N Stratis-Cullum

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