PMID: 11311857Apr 20, 2001Paper

Incorporation of 1-(14)C linoleic acid in rat liver nuclei and chromatin fractions

The International Journal of Biochemistry & Cell Biology
M Marmunti, A Catalá

Abstract

The incorporation of 1-(14)C linoleic acid in several chromatin fractions of rat liver nuclei was investigated using two different procedures: (1) rat liver nuclei were incubated with ATP, CoASH, Mg(++) and 1-(14)C linoleic acid. After 40 min at 37 degrees C the chromatin obtained by sonication of nuclei suspended in 0.25 M sucrose was fractionated by differential sedimentation; (2) chromatin fractions obtained by differential sedimentation were incubated separately with ATP, CoASH, Mg(++) and 1-(14)C linoleic acid 40 min at 37 degrees C in order to characterize the fatty acid incorporation in isolated chromatin. A comparative study of the incorporation of 1-(14)C linoleic acid in microsomes and nuclei isolated from rat liver is also presented for the purpose of comparison. Linoleic acid was incorporated into nuclear lipids as well as in chromatin fractions. The fatty acid incorporation was stimulated considerably in the acylation system when compared to control, it appears to be highly dependent on the state of condensation of chromatin, being barely detectable in the lowest density fraction. The major proportion of 1-(14)C linoleic acid was found in phospholipids and in a lesser proportion it remained esterified to triglyceri...Continue Reading

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Oct 24, 1998·The International Journal of Biochemistry & Cell Biology·M Marmunti, A Catalá

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Citations

Jan 26, 2002·The International Journal of Biochemistry & Cell Biology·David R JonesNullin Divecha

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