Incorporation of cofilin into rods depends on disulfide intermolecular bonds: implications for actin regulation and neurodegenerative disease.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
B W BernsteinJames R Bamburg

Abstract

Rod-shaped aggregates ("rods"), containing equimolar actin and the actin dynamizing protein cofilin, appear in neurons following a wide variety of potentially oxidative stress: simulated microischemia, cofilin overexpression, and exposure to peroxide, excess glutamate, or the dimer/trimer forms of amyloid-β peptide (Aβd/t), the most synaptotoxic Aβ species. These rods are initially reversible and neuroprotective, but if they persist in neurites, the synapses degenerate without neurons dying. Herein we report evidence that rod formation depends on the generation of intermolecular disulfide bonds in cofilin. Of four Cys-to-Ala cofilin mutations expressed in rat E18 hippocampal neurons, only the mutant incapable of forming intermolecular bonds (CC39,147AA) has significantly reduced ability to incorporate into rods. Rod regions show unusually high oxidation levels. Rods, isolated from stressed neurons, contain dithiothreitol-sensitive multimeric forms of cofilin, predominantly dimer. Oligomerization of cofilin in cells represents one more mechanism for regulating the actin dynamizing activity of cofilin and probably underlies synaptic loss.

References

Dec 13, 1988·Biochemistry·K A GiulianoJ R Bamburg
Jul 11, 1998·The Journal of Biological Chemistry·T L Vanden HoekP T Schumacker
Nov 28, 2000·Cell Motility and the Cytoskeleton·B W BernsteinJ R Bamburg
Oct 27, 2001·The Journal of Biological Chemistry·J PfannstielH Faulstich
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert E CampbellRoger Y Tsien
Mar 18, 2004·Antioxidants & Redox Signaling·John E Baker
Dec 21, 2004·Nature Neuroscience·James P ClearyKaren H Ashe
Jan 27, 2005·The Journal of Biological Chemistry·Yan WangKensaku Mizuno
Oct 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Dong-Hyuk JangBong-Kiun Kaang
Dec 13, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michael T MaloneyJames R Bamburg
Jun 2, 2006·American Journal of Physiology. Cell Physiology·Barbara W BernsteinJames R Bamburg
Jul 19, 2006·Cell Motility and the Cytoskeleton·Dmitry PavlovEmil Reisler
Sep 4, 2007·Progress in Brain Research·Thomas G Ohm
Nov 30, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Boyan K GarvalovFrank Bradke
Nov 26, 2008·The Journal of Cell Biology·Christian FrantzDiane L Barber
Dec 17, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Holly J CarlisleMary B Kennedy
Apr 3, 2009·Molecular Biology of the Cell·Jun-Sub KimGary M Bokoch
Jun 23, 2009·Journal of Alzheimer's Disease : JAD·Richard C DavisJames R Bamburg
Oct 16, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ineka T WhitemanClaire Goldsbury
Dec 22, 2009·The Journal of Biological Chemistry·Laurie S MinamideJames R Bamburg
Jan 22, 2010·Current Alzheimer Research·J R BamburgO Wiggan
Apr 21, 2010·Brain : a Journal of Neurology·Jessica M Mc DonaldUNKNOWN Medical Research Council Cognitive Function and Ageing Study
May 21, 2010·Biophysical Journal·Hongxia ZhaoPekka Lappalainen
Jun 26, 2010·Current Protocols in Cytometry·Bhaskar S Mandavilli, Michael S Janes
Dec 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Karl Herrup
Feb 8, 2011·Journal of Alzheimer's Disease : JAD·Ian T MarsdenJames R Bamburg

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Citations

Sep 5, 2013·The Journal of Biological Chemistry·Bianca SchulteYvonne Samstag
Dec 3, 2014·Molecular and Cellular Neurosciences·Irina SurguchevaAndrei Surguchov
Oct 15, 2013·Immunological Reviews·Yvonne SamstagGuido H Wabnitz
Oct 30, 2013·Journal of Neurochemistry·Timothy M Gomez, Paul C Letourneau
Dec 3, 2014·Biochimica Et Biophysica Acta·Manuela GellertChristopher Horst Lillig
Aug 13, 2013·Nucleus·Brittany J Belin, R Dyche Mullins
Oct 19, 2016·Cytoskeleton·Carlos WilsonGiasuddin Ahmed
Feb 14, 2016·Cytoskeleton·James R Bamburg, Barbara W Bernstein
Oct 17, 2018·PloS One·Seok Joon WonRaymond A Swanson
Oct 9, 2019·Journal of Alzheimer's Disease : JAD·David E Kang, Jung A Woo
Dec 26, 2018·Oxidative Medicine and Cellular Longevity·Claudia UrbainskyChristopher Horst Lillig
Feb 6, 2020·International Journal of Molecular Sciences·Silvia PelucchiElena Marcello
Mar 27, 2019·Communications Biology·Jung-A A WooDavid E Kang
Apr 7, 2020·Scientific Reports·Diana C Muñoz-LassoPilar Gonzalez-Cabo
Sep 4, 2013·Acta Crystallographica. Section D, Biological Crystallography·Marta KlejnotMichael F Olson
Dec 17, 2020·Frontiers in Cell and Developmental Biology·Qiang WangHaifa Qiao
Dec 8, 2020·Frontiers in Cell and Developmental Biology·Youssif Ben ZablahZhengping Jia
Mar 24, 2020·Journal of Proteome Research·Katrin BrenigGereon Poschmann
Jan 12, 2020·Communications Biology·Jung-A A WooDavid E Kang
May 1, 2021·Biology·Malgorzata KlocRafik M Ghobrial
May 15, 2021·Scientific Reports·Lisa Marie WalterPeter Claus
Jul 14, 2021·Biochemical and Biophysical Research Communications·Vibha KaushikPankaj Goyal
Aug 7, 2021·Brain Sciences·Tamara Lapeña-LuzónPilar Gonzalez-Cabo

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