PMID: 9161010Jun 1, 1997Paper

Incorporation of copper into the yeast Saccharomyces cerevisiae. Identification of Cu(I)--metallothionein in intact yeast cells

Journal of Inorganic Biochemistry
A Presta, M J Stillman

Abstract

Copper is an essential metal ion to many living organisms, including mammals, as it mediates a wide variety of important biochemical processes. At elevated concentrations, copper is extremely toxic to host cells. This paradoxical nature of copper has necessitated a highly regulated procedure for its cellular accumulation, transport, and excretion. One important group of proteins involved in eukaryotic copper speciation is the protein metallothionein. Luminescence microscopy data, emission, and circular dichroism spectral data are reported as copper is incorporated into metallothionein by the yeast Saccharomyces cerevisiae. These techniques provide information on the mechanism of copper uptake by S. cerevisiae. A two-stage kinetic mechanism for the uptake of copper from the growth medium by the yeast cells is observed. The first stage displays an uptake rate that is dependent on the initial copper concentration of the growth medium, and lasts for approximately 6 h. The second stage has a slower rate of copper uptake than the first, but the kinetics are independent of the initial copper concentration. Emission spectra recorded directly from the intact yeast cells (at 77 K) show that the cellular incorporation of copper proceeds v...Continue Reading

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Citations

May 24, 2005·Fungal Genetics and Biology : FG & B·Minoru Chikamori, Kazutaka Fukushima
Mar 27, 2003·Chemosphere·E López Errasquín, C Vázquez
Nov 24, 2004·Biochemical and Biophysical Research Communications·Kelly E Rigby, Martin J Stillman
Apr 14, 2009·Toxicology and Applied Pharmacology·Takamitsu MiyayamaYasumitsu Ogra
Jun 16, 2016·Metallomics : Integrated Biometal Science·Mireia FarrésRomà Tauler
Apr 7, 2005·Journal of Basic Microbiology·Stefan FujsMartin Batic
Jun 11, 2020·Antioxidants·Stefanie D BoydDuane D Winkler
Mar 17, 2011·Metallomics : Integrated Biometal Science·Takamitsu MiyayamaYasumitsu Ogra

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