Incorporation of extra amino acids in peptide recognition probe to improve specificity and selectivity of an electrochemical peptide-based sensor

Analytica Chimica Acta
Anita J ZaitounaRebecca Y Lai

Abstract

We investigated the effect of incorporating extra amino acids (AA) at the n-terminus of the thiolated and methylene blue-modified peptide probe on both specificity and selectivity of an electrochemical peptide-based (E-PB) HIV sensor. The addition of a flexible (SG)3 hexapeptide is, in particular, useful in improving sensor selectivity, whereas the addition of a highly hydrophilic (EK)3 hexapeptide has shown to be effective in enhancing sensor specificity. Overall, both E-PB sensors fabricated using peptide probes with the added AA (SG-EAA and EK-EAA) showed better specificity and selectivity, especially when compared to the sensor fabricated using a peptide probe without the extra AA (EAA). For example, the selectivity factor recorded in the 50% saliva was ∼2.5 for the EAA sensor, whereas the selectivity factor was 7.8 for both the SG-EAA and EK-EAA sensors. Other sensor properties such as the limit of detection and dynamic range were minimally affected by the addition of the six AA sequence. The limit of detection was 0.5 nM for the EAA sensor and 1 nM for both SG-EAA and EK-EAA sensors. The saturation target concentration was ∼200 nM for all three sensors. Unlike previously reported E-PB HIV sensors, the peptide probe functi...Continue Reading

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Citations

Feb 4, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Alexandra PlácidoCristina Delerue-Matos
Oct 18, 2016·Bioconjugate Chemistry·Alejandra TelloHumberto Gomez
Mar 5, 2021·Biosensors & Bioelectronics·Veronika VanovaVojtech Adam
Feb 7, 2017·Journal of Electroanalytical Chemistry·Samuel S Hinman, Quan Cheng
Feb 8, 2018·Analytical Chemistry·Julia PiccoliJason J Davis

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