PMID: 6990992May 28, 1980Paper

Incorporation of hydrogen atoms from deuterated water and stereospecifically deuterium-labeled nicotin amide nucleotides into fatty acids with the Escherichia coli fatty acid synthetase system

Biochimica Et Biophysica Acta
K SaitoT Yamakawa

Abstract

The mechanism of hydrogen incorporation into fatty acids was investigated with intact Escherichia coli cells, a crude enzyme preparation and purified reductases of fatty acid synthetase system. The distributions of deuterium atoms incorporated into fatty acids from 2H2O and stereospecifically deuterium-labeled NADPH or NADH were determined by mass spectrometry. When E. coli was grown in 2H2O, almost every hydrogen atom of cellular fatty acids was incorporated from the medium. When fatty acids were synthesized from acetyl-CoA, malonyl-CoA and NADPH in the presence of a crude enzyme preparation of either E. coli or Bacillus subtilis, almost every hydrogen atom was also incorporated from the medium. In contrast to these results, purified beta-ketoacyl acyl carrier reductase directly transferred the HB hydrogen of NADPH to beta-ketoacyl acyl carrier protein, and purified enoyl acyl carrier protein reductase also transferred the HB hydrogen of NADPH and NADH directly to enoyl acyl carrier protein. In the crude enzyme preparation of E. coli, we found high activities which exchanged the HB hydrogen of NADPH with the deuterium of 2h2o. the conflicting results of the origin of hydrogen atoms of fatty acids mentioned above are explained ...Continue Reading

References

Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T K ChangJ H Richards
Jan 1, 1973·Annual Review of Biochemistry·J J Volpe, P R Vagelos

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Citations

Jan 20, 2009·Cellular and Molecular Life Sciences : CMLS·R P Massengo-Tiassé, J E Cronan
Jul 21, 2009·Proceedings of the National Academy of Sciences of the United States of America·Xinning ZhangAlex L Sessions
May 26, 2015·Frontiers in Microbiology·Sandra M HeinzelmannMarcel T J van der Meer
Apr 18, 2015·FEMS Microbiology Letters·Sandra M HeinzelmannMarcel T J van der Meer
Oct 11, 2014·Applied Microbiology and Biotechnology·Selma MaricThomas Günther Pomorski
Jul 26, 2016·Frontiers in Microbiology·William D LeavittAlexander S Bradley
Aug 18, 2016·Frontiers in Microbiology·Magdalena R OsburnAlex L Sessions
Aug 13, 2020·Environmental Microbiology Reports·Weichao WuKai-Uwe Hinrichs

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