PMID: 7018587May 22, 1981Paper

Incorporation of large quantities of phospholipid by net de novo synthesis in cytoplasmic membrane vesicles of Escherichia coli

Biochimica Et Biophysica Acta
E J Demant

Abstract

De novo phospholipid synthesis has been studied in cytoplasmic membrane vesicles of Escherichia coli in the presence of sn-glycerol 3-phosphate, CTP, serine and acyl-CoA. Continuous addition of acyl-CoA was found to result in phospholipid biosynthesis during more than 4 h of incubation and phospholipid corresponding to 3--4-times the total amount of lipid originally present was synthesized under these conditions. The major biosynthetic products were phosphatidylglycerol, cardiolipin and phosphatidic acid. The composition of the accumulated lipid could to some extent be varied by adding the acyl-CoA to the vesicles at different rates. The newly synthesized lipid was found to be present in a population of membrane vesicles (80% of the total) which had a decreased density in comparison to the starting material. Electron microscopy of negatively stained preparations before and after incubation indicated that the vesicular nature of the cytoplasmic membrane fragments was preserved throughout incubation. A minor population of vesicles, the density of which was unchanged during incubation, contained little de novo synthesized lipid. The activity of the sn-glycerol-3-phosphate acyltransferase decreased during incubations to 25% in vesi...Continue Reading

References

Apr 1, 1979·European Journal of Biochemistry·E J DemantL L van Deenen
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·K E Langley, E P Kennedy
Jun 16, 1977·Biochimica Et Biophysica Acta·R M TeatherP Overath
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J E Rothman, E P Kennedy
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E Hawrot, E P Kennedy
May 1, 1975·European Journal of Biochemistry·M KitoS Sawada
Dec 1, 1975·Biochimica Et Biophysica Acta·H van HeerikhuizenB Witholt
Feb 14, 1972·Biochimica Et Biophysica Acta·J E Cronan, P R Vagelos
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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