Incorporation of lysyl-tRNA synthetase into human immunodeficiency virus type 1

Journal of Virology
Shan CenL Kleiman

Abstract

During human immunodeficiency virus type 1 (HIV-1) assembly, tRNA(Lys) isoacceptors are selectively incorporated into virions and tRNA(Lys)3 is used as the primer for reverse transcription. We show herein that the tRNA(Lys)-binding protein, lysyl-tRNA synthetase (LysRS), is also selectively packaged into HIV-1. The viral precursor protein Pr55gag alone will package LysRS into Pr55gag particles, independently of tRNA(Lys). With the additional presence of the viral precursor protein Pr160gag-pol, tRNA(Lys) and LysRS are both packaged into the particle. While the predominant cytoplasmic LysRS has an apparent M(r) of 70,000, viral LysRS associated with tRNA(Lys) packaging is shorter, with an apparent M(r) of 63,000. The truncation occurs independently of viral protease and might be required to facilitate interactions involved in the selective packaging and genomic placement of primer tRNA.

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Citations

Nov 1, 2002·Reviews in Medical Virology·David E Ott
Oct 26, 2012·The Journal of Biological Chemistry·Varun DewanKarin Musier-Forsyth
Sep 3, 2004·Nucleic Acids Research·Jennifer T MillerStuart F J Le Grice
Dec 17, 2009·Nucleic Acids Research·Cheryl BolingerKathleen Boris-Lawrie
Oct 20, 2006·Journal of Virology·Monika KaminskaMarc Mirande
Dec 3, 2010·Journal of Virology·Christopher P JonesKarin Musier-Forsyth
Nov 20, 2002·Journal of Virology·Shan CenLawrence Kleiman
Jul 30, 2003·Journal of Virology·Nathan J KellyCasey D Morrow
Apr 14, 2005·Journal of Virology·Cagan GurerJeremy Luban
Feb 22, 2012·Biochemistry. Biokhimii︠a︡·E V SmirnovaP A Kamenski
Mar 21, 2006·Retrovirology·Alan W CochraneAndrew J Mouland
Dec 17, 2009·RNA·Mariana Pavon-EternodLawrence Kleiman
Nov 22, 2013·Advances in Virology·Sanjeev K Anand, Suresh K Tikoo
Apr 27, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sang Gyu ParkSunghoon Kim
Nov 17, 2009·FEBS Letters·Lawrence KleimanKarin Musier-Forsyth
Jun 16, 2012·Virus Research·Jenan Saadatmand, Lawrence Kleiman
Feb 8, 2008·Virus Research·Truus E M Abbink, Ben Berkhout
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Apr 8, 2014·Wiley Interdisciplinary Reviews. RNA·Yan Ling Joy PangSusan A Martinis
Jun 5, 2008·FEMS Microbiology Reviews·Corinne D Hausmann, Michael Ibba
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Christopher P JonesKarin Musier-Forsyth

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