Incorporation of metabolites of 2'-fluoro-5-iodo-1-beta-D-arabinofuranosylcytosine into deoxyribonucleic acid of neoplastic and normal mammalian tissues

Biochemical Pharmacology
A J GrantF S Philips

Abstract

The radioactivity of 14C-labeled 2'-fluoro-5-iodo-1-beta-D-arabinofuranosylcytosine ([2-14C]-FIAC), a new and potent antiherpetic agent, was shown previously to be incorporated into the DNA fractions of mammalian and neoplastic tissues. The present work was undertaken to learn the nature of the incorporated moieties. Enzyme degradation of highly purified DNA from the small intestine of mice treated with [2-14C]FIAC and analysis of the resulting nucleosides failed to reveal the presence of unchanged FIAC. Rather, three metabolites were found, namely, the 2'-fluoro-1-beta-D-arabinofuranosyl nucleosides of cytosine (FAC), thymine (FMAU) and 5-iodouracil (FIAU). Labeled metabolites of FIAC were also found in the DNA isolated from P815 leukemic cells in mice given [2-14C]FIAC. It is of interest to note that FMAU, FIAU and FAC are, like FIAC, potent antiherpetic agents.

References

Jan 15, 1979·Biochemical and Biophysical Research Communications·S L Commerford, D D Joel
May 1, 1980·Antimicrobial Agents and Chemotherapy·C LopezJ J Fox

Citations

Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B C TennantJ L Gerin
Jun 1, 1996·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J M ColacinoF C Richardson
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·E BorrelliR M Evans
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·F C RichardsonR R Bowsher
Sep 1, 1987·Antimicrobial Agents and Chemotherapy·T C ChouJ J Fox

Related Concepts

Fiacitabine, (alpha-D)-isomer
Metazoa
Antiviral Agents
High Pressure Liquid Chromatography Procedure
Cytonal
DNA, Double-Stranded
DNA, Neoplasm
Mice, Inbred Strains
Mouse, Swiss

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.