PMID: 698208Sep 11, 1978Paper

Incorporation of N-acetylglucosamine from UDP-N-acetylglucosamine into proteins and lipid intermediates in microsomal and Golgi membranes from rat liver

Biochimica Et Biophysica Acta
A Bergman, G Dallner

Abstract

Rough and smooth microsomes and Golgi membranes incorporate N-acetylglucosamine from UDP-N-acetylglucosamine into endogenous protein acceptors. A lipid intermediate of the dolichol phosphate type participates in this transfer reaction in the case of both microsomal subfractions, but the nature of lipid glycosylation is different in these two fractions. Glucosamine transfer in Golgi membranes does not appear to involve a lipid intermediate. In contrast to the results obtained under in vivo conditions, no glucosamine label is recovered in nascent ribosomal proteins or on luminal secretory proteins after incubation in vitro. Proteolysis of intact vesicles of the subfractions removes glycosylated dolichol phosphate and protein acceptors to various extents and interferes with transferase activities. This finding suggests the possiblity that glycosylation at the cytoplasmic side of the membrane of the endoplasmic reticulum may involve a system separate from that acting at the luminal side of the same membrane.

References

Dec 29, 1975·Biochimica Et Biophysica Acta·J W Depierre, G Dallner
Mar 1, 1977·The Journal of Cell Biology·O S Nilsson, G Dallner
Jan 1, 1976·Annual Review of Biochemistry·C J Waechter, W J Lennarz
Jul 20, 1976·Biochimica Et Biophysica Acta·P ZattaD A Vessey
Jan 31, 1975·Molecular and Cellular Biochemistry·J Molnar
Nov 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·A J ParodiH Carminatti
May 1, 1973·The Biochemical Journal·W H Evans, J W Gurd
Nov 6, 1969·Biochemical and Biophysical Research Communications·J MolnarH Chao
Aug 15, 1972·FEBS Letters·G DallnerL F. Leloir

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Citations

Sep 11, 1978·Biochimica Et Biophysica Acta·E L AppelkvistG Dallner
May 6, 1981·Biochimica Et Biophysica Acta·K E Creek, D J Morré
Oct 1, 1981·Archives of Biochemistry and Biophysics·J A Hanover, W J Lennarz
Apr 1, 1980·Experimental and Molecular Pathology·I EggensG Dallner
Jan 1, 1985·Progress in Lipid Research·J W RipK K Carroll

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