PMID: 3320054Dec 1, 1987Paper

Incorporation of nascent myosin heavy chains into thick filaments of cardiac myocytes in thyroid-treated rabbits

The Journal of Cell Biology
M P Wenderoth, B R Eisenberg

Abstract

A monoclonal antibody (mAb 37) specific for alpha-myosin heavy chain (alpha-MHC) is used to follow the spatial and temporal incorporation of alpha-MHC into rabbit left ventricular myocytes. The expression of the two adult cardiac MHC genes, alpha and beta, is regulated by manipulating the thyroid hormone level of the animal. 10 wk on a propylthiouracil diet down-regulates expression of alpha-MHC to near 0%. alpha-MHC gene expression is up-regulated by injecting L-triiodothyronine (100 micrograms/kg per d) for 1-4 d. This protocol provides a means by which to follow the redistribution pattern of alpha-MHC within the myocyte in vivo. A uniform distribution of immunofluorescent signal is seen within every myocyte throughout the left ventricle. Ultracryomicrotomy without fixation is used to obtain sections for immunogold-electron microscopy. To quantify the immunogold method the density of gold-labeled antibody per unit of area tissue is determined for various regions of the sarcomere. Tissue from normal and 2-wk baby has a uniform distribution of gold density along the length of the A band. The average gold density of the A band increases with days of thyroid injection from 38 +/- 4 grains/micron 2 (n = 2 animals) (mean +/- SE) at...Continue Reading

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Jan 1, 1993·Cell Motility and the Cytoskeleton·K Imanaka-YoshidaJ W Sanger
Jan 1, 1990·Molecular Neurobiology·H F Epstein
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J E Lisman, M A Goldring
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Jun 1, 1991·Journal of Muscle Research and Cell Motility·A D SaadD A Fischman

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