PMID: 11607117Nov 1, 1990Paper

Incorporation of Paramecium axonemal tubulin into higher plant cells reveals functional sites of microtubule assembly

Proceedings of the National Academy of Sciences of the United States of America
M VantardA. M. Lambert

Abstract

Incorporation of Paramecium axonemal tubulin into lysed endosperm cells of the higher plant Haemanthus enabled us to identify sites of microtubule assembly. This exogenous Paramecium tubulin could be traced by specific antibodies that do not stain endogenous plant microtubules. Intracellular copolymerization of protozoan and higher plant tubulins gave rise to hybrid polymers that were visualized by immunofluorescence and by immunoelectron microscopy. The addition of exogenous tubulin revealed many free ends of endogenous microtubules that were competent to assemble ciliate tubulin. The functional roles of the nuclear surface and the equatorial region of the phragmoplast as plant microtubule-organizing centers, which were revealed by the intense incorporation of exogenous tubulin, are discussed. These data shed light on the present debate on higher plant microtubule organizing centers.

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Citations

Jan 1, 1992·Cell Motility and the Cytoskeleton·E A Smirnova, A S Bajer
Dec 22, 1999·European Journal of Cell Biology·V Stoppin-MelletA M Lambert
Feb 1, 1993·Current Opinion in Cell Biology·A M Lambert
Oct 20, 2001·Current Opinion in Plant Biology·R Nishihama, Y Machida
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D ZhangP K Hepler
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Ben Scheres, Philip N. Benfey
Apr 27, 2011·Tsitologiia·N V ShaminaI V Shevchenko
Jun 18, 2004·The Plant Journal : for Cell and Molecular Biology·Catherine Wicker-PlanquartMarylin Vantard
Feb 1, 1994·Current Opinion in Cell Biology·H Shibaoka, R Nagai
Feb 1, 1991·Current Opinion in Cell Biology·C J Staiger, C W Lloyd
Nov 1, 2013·Current Opinion in Plant Biology·Yuh-Ru Julie Lee, Bo Liu
Apr 1, 1991·Current Opinion in Cell Biology·S M Wick
Jun 8, 2000·Microscopy Research and Technique·J CanadayA C Schmit
Mar 7, 2001·Plant & Cell Physiology·S SonobeT Shimmen
Mar 22, 1996·Cell·L A Staehelin, P K Hepler

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