Incorporation of phytosterols in human keratinocytes. Consequences on UVA-induced lipid peroxidation and calcium ionophore-induced prostaglandin release

Chemistry and Physics of Lipids
M P Mora-RanjevaI Muller

Abstract

We have designed experimental conditions allowing the replacement of 50% of cholesterol of human keratinocytes (SVK14 line) with sitosterol or stigmasterol without affecting cellular viability. We have investigated the influence of incorporating phytosterol on the ultraviolet-A-induced formation of lipid-peroxidation products (thiobarbituric reactive substances (TBARS)) in these cells. Our results show that ultraviolet-A-induced lipid peroxidation depends on the nature of the phytosterol. Sitosterol induces a significant decrease (-30%) of TBARS relative to the control whereas stigmasterol markedly increases lipid peroxidation (+70%). We have also studied the effect of plant sterols on prostaglandin release by using the Ca(2+) ionophore A23187 as an in vitro model of the inflammation induced by UVA radiation. We show that in the presence of 50% of phytosterol (particularly stigmasterol), the release of prostaglandin (6-ketoPG(1alpha), PGE(2)) is increased compared to untreated cells. This pro-inflammatory effect of phytosterols is correlated with a loss of the regulation of the intracellular Ca(2+) concentration.

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Citations

Apr 30, 2013·Journal of Photochemistry and Photobiology. B, Biology·Anshu Rastogi, Pavel Pospíšil
Oct 10, 2009·Nutrition·Gianna FerrettiVirginia Bicchiega
Mar 5, 2010·Journal of Cardiovascular Pharmacology and Therapeutics·Christos S DerdemezisMoses S Elisaf
Aug 20, 2017·Naunyn-Schmiedeberg's Archives of Pharmacology·Cristiani Isabel Banderó WalkerGabriela Trevisan

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