May 1, 1976

Incorporation of proline into prodigiosin by a Put mutant of Serratia marcesens

Applied and Environmental Microbiology
D V LimR P Williams

Abstract

A Put mutant of Serratia marcescens, deficient in proline oxidase and therefore unable to degrade proline, was used to assay for an enzymatic reaction responsible for incorporation of proline into prodigiosin. The reaction had a pH optimum of 7.5 and a Km of 1.1 X 10(-4) M at 27 C. At temperatures above 27 C, the velocity of the reaction decreased with increasing temperature and little activity was detected at 42 C. Activity of the enzyme was directly proportional to the quantity of pigment formed and was inhibited by thioproline, a substrate analog. These data suggested the presence of a unique and specific enzyme in the biosynthetic pathway for prodigiosin.

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Mentioned in this Paper

Bacterial Proteins
Serratia marcescens
Oxidoreductases Acting on CH-NH Group Donors
Abufne
Proline
Biochemical Reaction
Mutant
Proline Dehydrogenase
Biosynthetic Pathways
Proline Dehydrogenase Deficiency

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