Incorporation of rhodopsin in laterally structured supported membranes: observation of transducin activation with spatially and time-resolved surface plasmon resonance

Biochemistry
S HeyseH Vogel

Abstract

Rhodopsin-transducin coupling was used as an assay to investigate a laterally patterned membrane reconstituted with a receptor and its G protein. It served as a model system to show the feasibility to immobilize G protein-coupled receptors on solid supports and investigate receptor activation and interaction with G proteins by one-dimensional imaging surface plasmon resonance. Supported membranes were formed by the self-assembly of lipids and rhodopsin from detergent solution onto functionalized gold surfaces. They formed micrometer-sized alternating regions of pure fluid phospholipid bilayers separated by bilayers composed of an outer phospholipid leaflet on a gold-attached inner thiolipid. Rhodopsin was found to incorporate preferentially into the phospholipid bilayer regions, whereas transducin was uniformly distributed over the entire outer surface of the supported patterned membrane. The influence of rhodopsin on the dark binding of transducin to lipid membranes was described quantitatively and compared with previously published data. Coupling reactions with transducin resembled closely the native system, indicating that the native functionality of rhodopsin was preserved in the supported membranes. The spatially varying p...Continue Reading

References

Jan 1, 1978·Analytical Biochemistry·P A CuypersH C Hemker
Apr 1, 1992·Journal of Bioenergetics and Biomembranes·J B Hurley
Jan 1, 1985·The Journal of Membrane Biology·R J LefkowitzM G Caron
Jun 12, 1986·Biochimica Et Biophysica Acta·H M McConnellA A Brian
Jan 1, 1987·The Journal of Membrane Biology·A Schleicher, K P Hofmann
May 1, 1995·Biophysical Journal·V M Unger, G F Schertler
Oct 1, 1995·Biophysical Journal·P NollertF Jähnig
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·T GudermannG Schultz
Feb 1, 1996·Quarterly Reviews of Biophysics·P B Garland
Dec 1, 1996·Biophysical Journal·S FelberK P Hofmann

❮ Previous
Next ❯

Citations

Nov 13, 2004·European Biophysics Journal : EBJ·Bruno Demé, Damien Marchal
May 19, 2006·European Biophysics Journal : EBJ·Peter J HardingAnthony Watts
Jul 6, 2007·European Biophysics Journal : EBJ·Claire Rossi, Joël Chopineau
Jun 2, 2005·Biochimica Et Biophysica Acta·Jasmina MinicJosep Samitier
Apr 28, 2004·Bioelectrochemistry·M R MoncelliS M Schiller
Nov 7, 1998·Biochimica Et Biophysica Acta·S HeyseH Vogel
Aug 1, 2000·Colloids and Surfaces. B, Biointerfaces·I HirataY Ikada
Jun 15, 1999·Trends in Biochemical Sciences·Z SalamonG Tollin
Dec 5, 2000·Current Opinion in Chemical Biology·S G Boxer
Jan 6, 2001·Journal of Biotechnology·Y ChengS D Evans
May 10, 2002·Journal of Biotechnology·K Busch, R Tampé
Sep 14, 2011·Journal of the American Chemical Society·Sophie RoizardHorst Vogel
May 12, 2009·Journal of the American Chemical Society·Michael RiskinItamar Willner
Sep 21, 2010·Physical Chemistry Chemical Physics : PCCP·Jacek Lipkowski
Jun 1, 2008·Biointerphases·Marcel LeuteneggerRudolf Robelek
Jun 1, 2008·Biointerphases·Louis X Tiefenauer, André Studer
Jun 16, 2010·Biosensors & Bioelectronics·Li WangShaojun Dong
Feb 2, 2010·Colloids and Surfaces. B, Biointerfaces·Jian ZhongYinlin Sha
Dec 5, 2015·Biophysical Journal·Yasushi TanimotoKenichi Morigaki
Apr 1, 2009·Journal of Structural Biology·Samuel TerrettazHorst Vogel
Jul 30, 2008·Methods : a Companion to Methods in Enzymology·Christophe DanelonHorst Vogel
Apr 5, 2003·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Per RiglerHorst Vogel
Feb 25, 2006·Angewandte Chemie·Michael J SpencelayhStephen D Evans
Oct 7, 2010·Small·Marta BallyBrigitte Städler
Jul 26, 2002·Analytical Biochemistry·N Madhusudhana RaoAnne L Plant
Apr 6, 2006·Chemistry and Physics of Lipids·Mojca BesenicarGregor Anderluh
Oct 29, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lutea A A de JongRainer Bischoff
Mar 13, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Henriette MozsolitsMarie-Isabel Aguilar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.