Incorporation of the HERG potassium channel in a mercury supported lipid bilayer

The Journal of Physical Chemistry. B
L BecucciRolando Guidelli

Abstract

The HERG potassium channel was incorporated in a mercury-supported tethered bilayer lipid membrane (tBLM) obtained by anchoring a thiolipid monolayer to the mercury surface and by self-assembling a lipid monolayer on top of it from a lipid film spread on the surface of an electrolyte solution. HERG was then incorporated in this tBLM from its micellar solution in Triton X-100, thus avoiding the use of vesicles in the preparation of the tBLM and of proteoliposomes in channel incorporation. The HERG "inward" current following a repolarization step was obtained by subtracting the current recorded upon addition of the specific inhibitor WAY from that recorded prior to this addition. This current was compared with that reported in the literature by the patch-clamp technique.

References

Oct 20, 1982·Biochimica Et Biophysica Acta·G D Eytan
Jan 18, 2003·Angewandte Chemie·Stefan M SchillerWolfgang Knoll
Mar 6, 2003·Journal of Colloid and Interface Science·T BaumgartW Knoll
Nov 13, 2003·Methods in Enzymology·Chris van der DoesArnold J Driessen
Apr 28, 2004·Bioelectrochemistry·M R MoncelliS M Schiller
Dec 1, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·Agnieszka Zebrowska, Paweł Krysiński
May 24, 2005·Biophysical Journal·Christian Horn, Claudia Steinem
Sep 22, 2005·Journal of the American Chemical Society·Lucia BecucciRolando Guidelli
Dec 8, 2006·Angewandte Chemie·Rudolf RobelekEva-Kathrin Sinner
Apr 11, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Lucia Becucci, Rolando Guidelli
Aug 4, 2007·The Journal of Physical Chemistry. B·Lucia BecucciRolando Guidelli

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Citations

Jul 29, 2009·Analytical and Bioanalytical Chemistry·Claus Hélix Nielsen
Nov 30, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Lina ZhongM Lane Gilchrist
Jan 28, 2014·Pharmaceuticals·Lucia Becucci, Rolando Guidelli
Aug 2, 2015·Chemistry and Physics of Lipids·C Patrick Collier
Feb 6, 2015·Biomedical Microdevices·Viksita VijayvergiyaJacob Schmidt
Jul 23, 2013·Biochimica Et Biophysica Acta·Lucia BecucciRolando Guidelli
Jan 3, 2015·Biochimica Et Biophysica Acta·Lucia BecucciRolando Guidelli
Dec 17, 2016·Membranes·Lucia Becucci, Rolando Guidelli
Jun 28, 2011·Physical Chemistry Chemical Physics : PCCP·Lucia BecucciRolando Guidelli
Jan 27, 2011·The Analyst·Sophie DemarcheJanos Vörös
Sep 17, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Monika Domińska, G J Blanchard

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