Incorporation, release and in-vitro antibacterial activity of liposomal aminoglycosides against Pseudomonas aeruginosa

The Journal of Antimicrobial Chemotherapy
Abdelwahab OmriM Poisson

Abstract

Amikacin, netilmicin and tobramycin were incorporated into either anionic or cationic liposomes prepared by sonication. The influence of lipid constituents (charges) on encapsulation efficiency was determined after lysis of vesicles by 0.2% (v/v) Triton X-100. The in-vitro activities of the liposomal aminoglycosides were evaluated against Pseudomonas aeruginosa by agar dilution and compared with free antibiotics. Normal human pooled sera, incubated at 37 degrees C, were supplemented with anionic or cationic liposomes containing known fixed concentrations of amikacin, netilmicin or tobramycin. At various time intervals (0-48 h), samples were taken and antibiotic concentrations determined by the enzyme multiplied immunoassay technique (EMIT). The encapsulation efficiency of cationic liposomes (amikacin 17.1 +/- 1.55%, netilmicin: 5.63 +/- 1.13%, tobramycin 6.7 +/- 0.5%) was approximately 30% higher than that of anionic liposomes (amikacin 12.3 +/- 0.95%, netilmicin 4.0 +/- 0.06%, tobramycin 5.13 +/- 0.18%). Anionic and cationic liposomes in human serum still retained 79.13 +/- 4.04% and 82.71 +/- 2.6% of amikacin, 50.67 +/- 1.8% and 38.6 +/- 0.8% of netilmicin, and 89.09 +/- 1.0% and 88.93 +/- 0.4% of tobramycin, respectively, af...Continue Reading

Citations

Jan 1, 2011·International Journal of Pharmaceutical Investigation·Roonal L Jain, J P Shastri
Jul 28, 2013·Expert Opinion on Drug Delivery·Moayad AlhaririAbdelwahab Omri
Jan 1, 1998·Drug Delivery·G A NobesD Maysinger
May 4, 2012·Veterinary Immunology and Immunopathology·Ramasamy HarikrishnanMoon-Soo Heo
Nov 18, 2005·International Journal of Pharmaceutics·Clement MugabeAbdelwahab Omri
Nov 25, 2014·The Journal of Antimicrobial Chemotherapy·Venkata Saran SolletiAbdelwahab Omri
Oct 16, 2004·The Journal of Antimicrobial Chemotherapy·Ashraf A KadryMohsen A Bayomi
Mar 27, 2020·ACS Infectious Diseases·Azucena Gonzalez Gomez, Zeinab Hosseinidoust

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