PMID: 6414112Jul 1, 1983Paper

Increase during passage in culture in susceptibility of Syrian hamster embryonic cells to induction of chromosome aberrations by 7,12-dimethylbenz(a)-anthracene

Toxicology Letters
Y NishiN Inui

Abstract

Studies were made on chromosome aberrations in Syrian hamster embryonal fibroblasts (SHEF) at early and late passages in culture. Aberrations were induced with a potent indirect clastogen, 7,12-dimethylbenz(a)anthracene (DMBA). Results showed that cells at late passages were much more susceptible to this chemical than cells at early passages: the frequency of aberrant metaphases was usually less than 20% at the 2nd passage, but it gradually increased with increase in the number of culture passages, reaching 50% or more at the 8th passage in one experiment and 6th-7th passage in another. Induction of chromosome aberrations was also examined in V79 cells co-cultured with hamster cells at early and late passages. V79 cells are known to have no drug-metabolizing enzyme activity. Results demonstrate that the frequency of chromosome aberrations of V79 cells was much greater in the presence of hamster cells at late passages than in the presence of cells at early passages.

References

May 1, 1979·Chemico-biological Interactions·T HirakawaS Takayama
Mar 1, 1979·Mutation Research·A MatsuokaM Ishidate
Dec 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·H V GelboinL Sachs
Mar 15, 1974·International Journal of Cancer. Journal International Du Cancer·E Huberman, L Sachs

❮ Previous
Next ❯

Citations

Mar 14, 2021·Environmental Pollution·Francisco Carlos da Silva JuniorSilvia Regina Batistuzzo de Medeiros

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.