Increase in Endogenous and Exogenous Cyclic AMP Levels Inhibits Sclerotial Development in Sclerotinia sclerotiorum

Applied and Environmental Microbiology
Jeffrey A Rollins, Martin B Dickman

Abstract

Growth and development of a wild-type Sclerotinia sclerotiorum isolate were examined in the presence of various pharmacological compounds to investigate signal transduction pathways that influence the development of sclerotia. Compounds known to increase endogenous cyclic AMP (cAMP) levels in other organisms by inhibiting phosphodiesterase activity (caffeine and 3-isobutyl-1-methyl xanthine) or by activating adenylate cyclase (NaF) reduced or eliminated sclerotial development in S. sclerotiorum. Growth in the presence of 5 mM caffeine correlated with increased levels of endogenous cAMP in mycelia. In addition, incorporation of cAMP into the growth medium decreased or eliminated the production of sclerotia in a concentration-dependent manner and increased the accumulation of oxalic acid. Inhibition of sclerotial development was cAMP specific, as exogenous cyclic GMP, AMP, and ATP did not influence sclerotial development. Transfer of developing cultures to cAMP-containing medium at successive time points demonstrated that cAMP inhibits development prior to or during sclerotial initiation. Together, these results indicate that cAMP plays a role in the early transition between mycelial growth and sclerotial development.

References

Apr 1, 1991·Journal of General Microbiology·G D RobsonA P Trinci
Jan 1, 1990·Annual Review of Biochemistry·S S TaylorW Yonemoto
Mar 2, 1973·Archiv für Mikrobiologie·D L Corsini, D Le Tourneau
Sep 1, 1981·Microbiological Reviews·M L Pall
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·G H ChoiD L Nuss
Dec 1, 1994·Genes & Development·S GoldJ Kronstad
Apr 15, 1997·The EMBO Journal·E RegenfelderR Kahmann
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·S Gao, D L Nuss
Jan 1, 1996·Applied and Environmental Microbiology·V Warwar, M B Dickman

❮ Previous
Next ❯

Citations

Jan 3, 2001·Applied and Environmental Microbiology·J A Rollins, M B Dickman
Dec 20, 2012·Antioxidants & Redox Signaling·Noam AlkanDov Prusky
Sep 6, 2011·Applied and Environmental Microbiology·Hyo-jin KimMartin B Dickman
Apr 1, 2014·World Journal of Microbiology & Biotechnology·María Isabel FonsecaPedro Darío Zapata
Nov 29, 2016·Frontiers in Microbiology·Jie ZhongQian Zhou
Jan 10, 2017·Frontiers in Cellular and Infection Microbiology·Kunlong YangShihua Wang
Mar 26, 2003·Annual Review of Phytopathology·Nancy LeeJames W Kronstad
Apr 14, 2004·Molecular Plant-microbe Interactions : MPMI·Changbin ChenMartin B Dickman
Aug 23, 2005·FEMS Microbiology Letters·Dwayne D Hegedus, S Roger Rimmer
Jan 1, 2006·Molecular Plant Pathology·Melvin D BoltonBerlin D Nelson
Nov 4, 2015·Plant Signaling & Behavior·Akifumi SugiyamaHiroshi Sano
Oct 7, 2016·Frontiers in Microbiology·Wei WeiFang Peng
Jan 19, 2010·Molecular Plant Pathology·Zafer Dallal BashiMohammed H Borhan
Jun 30, 2018·Annual Review of Phytopathology·Liangsheng XuWeidong Chen
Apr 23, 2010·Journal of Proteome Research·Yue LiangNat N V Kav

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.