Increase in functional Ca2+ channels in cerebral smooth muscle with renal hypertension

Circulation Research
J M SimardK Tewari

Abstract

The hypothesis that availability of functional Ca2+ channels in vascular smooth muscle is augmented in hypertension was tested in basilar artery cells from Wistar rats exhibiting stable systolic blood pressure (BPsys) for 2 to 11 weeks after partial renal artery ligation (Goldblatt 2-kidney 1-clip [2K1C] model). Cells were freshly isolated and patch-clamped using a nystatin-perforated patch method. BPsys ranged from 110 to 280 mm Hg and correlated with normalized kidney mass. Macroscopic current-voltage curves were fit to a Boltzmann function to obtain maximum conductance (gmax), steepness and midpoint potential for the voltage dependence of activation (k and E1/2, respectively), and extrapolated reversal potential for the chord conductance (Erev). Linear regression of normalized conductance (ng(max)=g(max)/cell capacitance) versus BPsys for 103 cells indicated a strong relationship, with a slope of 0.0019 nS x pF(-1) x mm Hg(-1) (P<0.0001). Similar analysis of data from 35 other cells exposed to 500 nmol/L Bay K 8644 gave a slope of 0.0041 nS x pF(-1) x mm Hg(-1) (P=0.001). Voltage-dependent parameters, k, E1/2, and Erev, were not significantly related to BPsys. Single-channel measurements in cell-attached patches revealed tha...Continue Reading

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Citations

Jun 21, 2001·Hypertension Research : Official Journal of the Japanese Society of Hypertension·M Asano, Y Nomura
Oct 19, 2013·Journal of Physiology and Biochemistry·Cristina Porras-GonzálezMaría Angeles Muñoz-Sánchez
Dec 23, 2006·The Journal of Physiology·Elena NikitinaR Loch Macdonald
Feb 5, 2013·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Biny K JosephSung W Rhee
Jan 24, 2006·Vascular Pharmacology·Swapnil SonkusareNancy J Rusch
Jul 23, 2014·BioMed Research International·Christopher T ChanGrant R Drummond
Aug 1, 2006·American Journal of Hypertension·Wen-Ze WangPhilip Palade
Feb 5, 2008·American Journal of Hypertension·Robert H Cox, Irina M Lozinskaya
Dec 23, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Yasuo AiharaR Loch Macdonald
May 17, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Man-Jiang XieHong-Wei Cheng
Mar 14, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Jun-Hui XueMan-Jiang Xie

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