Increase in phospholamban content in mouse skeletal muscle after denervation

Journal of Muscle Research and Cell Motility
Masatoshi KomatsuMitsuhiko Yamada

Abstract

It is well-known that denervation of motor nerves induces atrophy and decreases contractile force of the skeletal muscle. However, it is not completely understood how denervation alters calcium handling in the skeletal muscle. We investigated the effect of denervation on the expression and function of proteins involved in calcium handling. Two weeks after denervation of the right sciatic nerve in mice, we observed a significant decrease in mass and cross-sectional area of the ipsilateral tibialis anterior (TA) and flexor digitorum brevis (FDB) muscles. Also, we observed a significant decrease in the specific tetanus contractile force in the ipsilateral TA muscle. Calcium imaging of the ipsilateral FDB showed that the peak twitch and tetanus calcium concentrations were significantly decreased due to a decrease in calcium content of the sarcoplasmic reticulum (SR). Denervation reduced the maximum rate of sarcoplasmic/endoplasmic calcium ATPase (SERCA) activity. Interestingly, the amount of phospholamban (PLN), but not its transcripts, was increased in the ipsilateral vs. contralateral side after denervation, suggesting that denervation impairs constitutive regulation of PLN. Immunohistochemical analysis revealed increased PLN in ...Continue Reading

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Citations

Feb 18, 2021·Journal of Muscle Research and Cell Motility·D Mejía-RaigosaJ C Calderón
May 20, 2021·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Renqiang YuanDelin Mo
Jun 4, 2021·Biochimica Et Biophysica Acta. General Subjects·Andrés F MilánJuan C Calderón

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BETA
protein assay
electrophoresis
PCR
ICE
ubiquitination

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