Increase in placental apolipoprotein D as an adaptation to human gestational diabetes.

Placenta
Ana NavarroJorge Tolivia

Abstract

The expression of apolipoprotein D (apo D), a lipocalin involved in defense mechanisms against oxidative stress, in placental tissue samples of pregnancies with gestational diabetes mellitus (GDM) was compared to non-diabetic controls. We have investigated the relationship of apo D with 4-HNE, a major propagation product of lipid peroxidation, in stressed tissues. We included 20 pregnant women with GDM and 30 women with normal ongoing pregnancies as the control group. Placentas were collected and frozen for Western blot or included in paraffin for immunohistochemistry. The intensity of immunostaining was higher for apo D and 4-HNE in GDM samples; however, the differences in expression between the groups was more intense for apo D. Positive signals for both antibodies was detected in the villous trophoblast and adventitia tunica around the large blood vessels for all groups. Specific immunostaining for apo D was noted in some mesenchymal and macrophagic-like cells and this signal increased in diabetic placentas. Densitometry analysis of Western blots showed no significant difference for 4-HNE, but was significantly more intense for apo D in diabetic women. The contradictory results for 4-HNE could be due to changes which are too...Continue Reading

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Citations

Feb 6, 2017·Biomarkers in Medicine·James Sm CuffeAnthony V Perkins
Jun 17, 2011·Antioxidants & Redox Signaling·Martha LappasAlicia Jawerbaum
Nov 14, 2020·Scientific Reports·Arthur T KopylovSergey G Morozov
Aug 8, 2021·International Journal of Molecular Sciences·Andrea Olmos-OrtizVerónica Zaga-Clavellina
Oct 26, 2021·Frontiers in Physiology·Diego Sanchez, Maria D Ganfornina

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Methods Mentioned

BETA
electrophoresis
MDA

Software Mentioned

NIH Image
SPSS

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