Increase in surface expression of ICAM-1, VCAM-1 and E-selectin in human cerebromicrovascular endothelial cells subjected to ischemia-like insults

Acta Neurochirurgica. Supplement
D StanimirovicJ Durkin

Abstract

Secondary ischemic brain injury has been shown to develop as a consequence of inflammation and vasogenic brain edema. In this study we show that inflammatory cytokines and simulated in vitro ischemia stimulate the surface expression of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1) and endothelial-leukocyte adhesion molecule-1 (E-selectin) in human cerebromicrovascular endothelial cells (HCEC) in culture. The levels of all three adhesion molecules were dramatically (3 to 10-fold) up-regulated by 4-24 hour exposure to the inflammatory cytokines. IL-1 beta (10-200 u/ml) or TNF alpha (50 200 u/ml), and by a 4 hour exposure to "simulated" in vitro ischemia, as determined by immunocytochemistry and ELISA. Following 24 hours of subsequent reperfusion, the expression of ICAM-1 and VCAM-1 was maintained at ischemia-induced levels, whereas E-selectin was no longer detectable. Both the cytokine- and ischemia-induced up-regulation of adhesion molecules were completely abolished by the transcriptional inhibitor, actinomycin D (10 micrograms/ml), and inhibited by the cycloxygenase (COX) inhibitor, indomethacin (300 microM). These findings implicate HCEC in the processes of leukocyte adhesion and recru...Continue Reading

Citations

Apr 19, 2017·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Seyed Esmaeil KhoshnamHadi Fathi Moghaddam
Jun 14, 2019·Frontiers in Neuroscience·Danielle N Edwards, Gregory J Bix
Dec 19, 2019·Frontiers in Immunology·Yijie WangAnwen Shao
Dec 20, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Jose A OspinaSue P Duckles
Jul 10, 2019·Frontiers in Pharmacology·Kyle MaloneChristian Waeber
May 10, 2017·Journal of Stroke·Seyed Esmaeil KhoshnamMaryam Farzaneh
May 12, 2021·Journal of Neuroinflammation·Benton MaglingerKeith R Pennypacker

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