Increase in the intramolecular disulfide bonding of alpha-A crystallin during aging of the human lens

Experimental Eye Research
L J Takemoto

Abstract

Aging of the normal human lens is accompanied by oxidation of protein sulfhydryl groups to disulfide groups. Although this has been known for many years, very little is known about the exact amino acid residues involved. In addition, almost nothing is known concerning the temporal sequence of this oxidative process over the lifetime of the individual. To address these two concerns for alpha-A crystallin, the polypeptide was purified from total proteins of the human lens, followed by digestion with lys-C endoprotease. Mass spectral analysis of the resulting fragments demonstrated that the two cysteine residues (cysteine-131 and cysteine-142) are present as a mixture of an intramolecular disulfide bond and free sulfhydryl groups. Reverse phase chromatography was used to resolve and quantitate the relative amounts of the two forms present in alpha-A crystallin from normal lenses of different age. Even in very young lenses (4 months and 5 months of age) there is significant oxidation of the two cysteine residues. However, the oxidative state of these two residues does not significantly change during the next approximately 27 years of age, after which there is an increase in the relative amount of intramolecular disulfide bonding. T...Continue Reading

Citations

Nov 21, 2008·Molecular and Cellular Biochemistry·Santosh R KanadeRam H Nagaraj
Mar 16, 2011·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R Michael, A J Bron
Nov 19, 2003·The Journal of Biological Chemistry·Om P SrivastavaKiran Srivastava
Apr 21, 2018·The FEBS Journal·Takumi TakataNoriko Fujii
Dec 16, 1998·Journal of Protein Chemistry·T X SunJ J Liang
Dec 4, 2019·Nature Structural & Molecular Biology·Christoph J O KaiserSevil Weinkauf
Feb 13, 1999·The Journal of Biological Chemistry·S R HansonM F Lou
Oct 24, 1998·The Journal of Biological Chemistry·S FuR Truscott
Jun 10, 2014·Acta Ophthalmologica·Ariana D GoertzLindsay A Nelson
Nov 11, 2019·Progress in Retinal and Eye Research·Kevin L ScheyRoger J W Truscott
Apr 15, 2021·Expert Review of Proteomics·Lee S Cantrell, Kevin L Schey
Feb 13, 2001·Journal of Molecular Biology·S J ChenJ J Liang
Dec 22, 1999·Biochemical and Biophysical Research Communications·N FujiiM Akaboshi
Sep 24, 1999·Biochemical and Biophysical Research Communications·N FujiiM Akaboshi
Jul 12, 2001·Experimental Eye Research·Z ZhangJ B Smith
Feb 14, 2002·Archives of Biochemistry and Biophysics·Christine Colvis, Donita Garland
Nov 14, 2002·Biochimica Et Biophysica Acta·Luis J del VallePere Garriga
Aug 2, 2003·Progress in Retinal and Eye Research·Marjorie F Lou
Aug 2, 2021·Experimental Eye Research·Eugene SerebryanyLiliana Quintanar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.