Increase in the molecular weight and radius of gyration of apocalmodulin induced by binding of target peptide: evidence for complex formation

FEBS Letters
Y IzumiH Yoshino

Abstract

Small-angle X-ray scattering was used to investigate a complex state of apocalmodulin induced by the binding of a Ca(2+)/calmodulin-dependent protein kinase IV calmodulin target site. Upon binding of the peptide, the molecular weight for apocalmodulin increased by 8.4%, which provides direct evidence for the formation of a calmodulin/target peptide complex. Comparison of the radius of gyration and Kratky plots of the apocalmodulin/peptide complex with those of apocalmodulin indicates that the overall conformation remains unchanged but the flexibility of the central linker decreases. An analysis of residue pairs between calmodulin and the target peptides suggests that the complex formation is induced by electrostatic interactions and subsequent van der Waals interactions.

References

Jan 1, 1991·Pharmacology & Therapeutics·A R MeansC D Rasmussen
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M KataokaD M Engelman
Oct 1, 1986·Journal of Inorganic Biochemistry·R H KretsingerL J Weissman
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·A Houdusse, C Cohen
Sep 1, 1995·Nature Structural Biology·H KuboniwaA Bax
Sep 1, 1995·Nature Structural Biology·B E FinnS Forsén
Jan 1, 1995·Trends in Biochemical Sciences·P JamesE Carafoli
Apr 1, 1994·Journal of Biochemistry·T KitaniH Fujisawa
Oct 1, 1996·Protein Science : a Publication of the Protein Society·D van der SpoelH J Vogel
Apr 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A R Rhoads, F Friedberg
Apr 1, 1998·Protein Science : a Publication of the Protein Society·M R Nelson, W J Chazin
Apr 7, 1999·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·M R Nelson, W J Chazin
Jun 22, 1999·The Journal of Biological Chemistry·P O TsvetkovA A Makarov
Jul 3, 1999·Physiological Reviews·L A JuradoH W Jarrett

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Citations

Feb 5, 2009·The Review of Scientific Instruments·Xinguo Hong, Quan Hao
Aug 31, 2002·Biophysical Journal·Andriyka L PapishHans J Vogel

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