Increase of a form of UDP-glucuronyltransferase glucuronizing various phenolic xenobiotics and the corresponding translatable mRNA in 3-methylcholanthrene-treated rat liver

Journal of Biochemistry
H Yokota, A Yuasa

Abstract

Induction of hepatic microsomal UDP-glucuronyltransferase activity toward various phenolic xenobiotics by 3-methylcholanthrene treatment of rats was observed, and the process of the induction was studied. We had previously purified a form of UDP-glucuronyltransferase (called GT-1) having a catalytic activity toward phenolic xenobiotics from liver microsomes of 3-methylcholanthrene-treated rats. The antibodies against GT-1 inhibited the enzyme activity toward those xenobiotics in liver microsomes, and bound to a single protein having a molecular weight of about 54,000 Da (same value as that of GT-1) among microsomal proteins on immunoblotting analysis. The amount of GT-1 protein in hepatic microsomes was found to be increased in close correspondence with the activity increase by 3-methylcholanthrene treatment, by immunoblotting analysis using an uninducible cytochrome P-450 reductase as a negative standard. It was shown by in vitro translation assays that the protein increase described above resulted from the enhancement of the level of translatable mRNA encoding for GT-1. Increases in the amount of the protein immunochemically corresponding to GT-1 in the microsomes from liver of phenobarbital-treated rats and from extrahepatic...Continue Reading

Citations

Jun 15, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Junya MatsumotoHiroshi Yokota
Mar 29, 2011·The Journal of Veterinary Medical Science·Taku MiyashoHiroshi Yokota
Jun 28, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Yuki TochigiHiroshi Yokota
Oct 26, 2002·Biochimica Et Biophysica Acta·Hirokazu SakamotoAkira Yuasa

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