PMID: 3765775May 1, 1986Paper

Increase of cellular hyaluronic acid synthesis following continuous exposure of cultured human fibroblasts (WI-38) to hydrocortisone

Zeitschrift für Gerontologie
D O Schachtschabel, G Sluke

Abstract

Continuous (long-term) exposure of cultured normal (diploid) human fibroblasts (WI-38) to hydrocortisone (1.4 X 10(-7) M) resulted, as originally described by Macieira-Coelho (1966) and Cristofalo (1970), in a stimulation of proliferative activity and an increase of population doublings. Stimulation of DNA-synthesis by hydrocortisone, as measured by 3H-thymidine incorporation, required the presence of serum in the culture medium. Analysis of the cellular glycosaminoglycan (GAG) pattern, as measured by 14C-glucosamine incorporation into the various GAG types (hyaluronic acid, heparan sulfate, chondroitin sulfate, dermatan sulfate) revealed a significant increase of cell-bound hyaluronic acid (it appears to be predominantly located at the cell surface or pericellular, since it is removable to a large extent by trypsin treatment), while the distribution pattern of sulfated GAGs did not exhibit a significant change. This increase of cellular hyaluronic acid synthesis was regarded largely as an adaptive response to hydrocortisone, since its removal from the culture medium of hydrocortisone pretreated cultures resulted in a significant decrease of cellular hyaluronic acid. Possible functions of cell-bound hyaluronic acid were suggest...Continue Reading

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