PMID: 2492679Jan 1, 1989Paper

Increase of extrachromosomal circular DNA in mouse 3T6 cells on perturbation of DNA synthesis: implications for gene amplification

Somatic Cell and Molecular Genetics
P SunnerhagenG Bjursell

Abstract

We have analyzed the amount of extrachromosomal double-stranded covalently closed circular nonmitochondrial DNA in mouse 3T6 cells by Southern blotting and electron microscopy. Treatment with 7,1-dimethylbenz[a]anthracene, known to promote amplification of integrated SV40 genomes, elevated the amount of circular DNA. Inhibition of DNA synthesis with hydroxyurea, earlier shown to enhance amplification of the cellular dihydrofolate reductase gene, resulted in yet higher levels. Thus, elevation of the frequency of gene amplification and generation of extrachromosomal circular DNA seem to accompany each other in the situations studied in this paper. Two other DNA synthesis inhibitors, aphidicolin and thymidine, had markedly lesser effects on circular DNA. The significance of these findings for the mechanism of gene amplification is discussed.

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Citations

Jan 1, 1991·Mutation Research·R B Painter, L N Kapp
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·M D Summers, S D Dib-Hajj
Feb 1, 1997·Human Molecular Genetics·F BoldogH A Drabkin
Jul 11, 1994·Nucleic Acids Research·N van LoonJ P Murnane
Aug 1, 1997·Journal of Molecular Evolution·T Oosumi, W R Belknap
Mar 14, 2018·Proceedings of the National Academy of Sciences of the United States of America·Dal-Hoe KooBikram S Gill
Dec 3, 2015·Expert Opinion on Drug Discovery·Guido Tomás RozenblumMartín Radrizzani
Sep 13, 2013·International Journal of Cancer. Journal International Du Cancer·Wei JiSongbin Fu
Mar 24, 2021·Minerva Surgery·Niccolò PetruccianiAntonio Iannelli

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