Increase of germ cell nuclear factor expression in globozoospermic Gopc-/- knockout mice

Andrology
M BizkarguenagaE Alonso

Abstract

Golgi-associated PDZ and coiled-coil motif-containing protein (GOPC) is a Golgi protein that plays a role in vesicular transport and intracellular protein trafficking and degradation. Mice deficient in GOPC protein have globozoospermia and are infertile. The germ cell nuclear factor (GCNF) is a member of the nuclear receptor superfamily which is expressed in male germ cells, from spermatocytes and spermatids, both in the nucleus and the acrosomal region. It is not known if its expression could be altered in Gopc-/- mice with defective acrosomes. The aim of the present work was to analyze the distribution of GCNF protein in spermatids of Gopc-/- knockout mice. We have analyzed the expression and distribution during spermatogenesis of GCNF and its deregulation in Gopc-/- mutant mice by RT-qPCR, Western blot, immunohistochemistry and immunogold. Germ cell nuclear factor was localized in the nucleus of all the cell types in the seminiferous tubules. Despite being a nuclear protein, it was also located in the acrosome and in the manchette of elongating spermatids. We have found that in the absence of GOPC, the expression of GCNF was increased in the nucleus of spermatocytes, mainly in leptotene, and in the nucleus and the manchette ...Continue Reading

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Citations

May 28, 2020·International Journal of Molecular Sciences·Maria E TevesPaulene Sapao
Oct 3, 2019·American Journal of Physiology. Cell Physiology·Zhenyu WangZhibing Zhang
Dec 7, 2021·Microscopy Research and Technique·Maider BizkarguenagaEdurne Alonso

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