Increase of glial fibrillary acidic protein fragments in the spinal cord of motor neuron degeneration mutant mouse

Brain Research
K FujitaY Nagata

Abstract

We analyzed protein fractions extracted from the spinal cord of the motor neuron degeneration (Mnd) mouse, a mutant that exhibits progressive degeneration of lower spinal motor neurons, by one- and two-dimensional polyacrylamide gel electrophoresis (PAGE) after solubilization of the tissue with medium containing sodium dodecyl sulfate (SDS)-urea during growth of the animal, in comparison with those of age-matched controls (C57BL/6). Several protein spots were detected around a region of pI 5.6-6.0 and molecular mass of 35-50 kDa in Mnd spinal cord tissue on the two-dimensional PAGE separation profile with Coomassie brilliant blue staining, while only a few spots around the same region were found in the control spinal cord. These spots were all immunoreactive with an antibody against glial fibrillary acidic protein (GFAP), a cytoskeleton filamentous protein specific to astroglial cells. The protein spot with molecular mass of 50 kDa showed immunoreactivity with anti-GFAP antibody, had a blocked amino-terminus, and is assumed to be intact GFAP. Several protein spots with slightly smaller molecular masses of 35 to 48 kDa lacked the head domain of the GFAP molecule as a result of cleavage at the 29th and 56th residues from the amin...Continue Reading

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Citations

Oct 31, 2002·Journal of Neuroscience Research·Tiziana MenniniCaterina Bendotti
Jul 4, 2001·Neuroreport·P BiginiT Mennini
Jun 27, 1998·Molecular and Chemical Neuropathology·Y NagataM Honda
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Feb 21, 2019·Nature Reviews. Neurology·Tyler B JohnsonJill M Weimer

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