Increase of INS-1 cell apoptosis under glucose fluctuation and the involvement of FOXO-SIRT pathway

Diabetes Research and Clinical Practice
Mikyung KimJeonghyun Park

Abstract

Variations of blood glucose level have been reported to be more harmful than sustained high glucose, but the effects on pancreatic β-cells have not yet been clarified. FOXO transcription factors are important for cell fate. We tried to clarify the effect of glucose variability on INS-1 cells, and the potential mechanisms related with FOXO-SIRT pathway. INS-1 cells were exposed to control, SHG (sustained high glucose) or IHG (intermittent high glucose) alternating every 12 h for 5 days. INS-1 cells in SHG showed lower apoptosis and higher GSIS than IHG. Deacetylated FOXO and binding with SIRT were higher in SHG than IHG. Administration of PI3K inhibitor and/or SIRT inhibitor increased apoptosis and decreased Mn-SOD and Bcl-2 in SHG. [corrected] IHG was more harmful to INS-1 cells than SHG. The degree of phosphorylation and acetylation of FOXO transcription factors were different between SHG and IHG, which might be one mechanism of increased INS-1 cell apoptosis in IHG.

References

Sep 30, 1993·The New England Journal of Medicine·UNKNOWN Diabetes Control and Complications Trial Research GroupC Siebert
Sep 28, 2002·Diabetes Care·James B MeigsUNKNOWN Framingham Offspring Study
Oct 24, 2002·The Journal of Biological Chemistry·Christian E WredeChristopher J Rhodes
May 30, 2003·Journal of Leukocyte Biology·Boudewijn M T Burgering, René H Medema
Feb 26, 2004·Cell·Maria Carla MottaLeonard Guarente
Jun 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hiroaki DaitokuAkiyoshi Fukamizu
Jul 29, 2006·European Journal of Cardiovascular Prevention and Rehabilitation : Official Journal of the European Society of Cardiology, Working Groups on Epidemiology & Prevention and Cardiac Rehabilitation and Exercise Physiology·Tomoko NakagamiKnut Borch-Johnsen
May 20, 2008·Molecular and Cellular Endocrinology·Zhi-Qiang HouGuang-Wei Li

❮ Previous
Next ❯

Citations

Jun 1, 2014·The Canadian Journal of Neurological Sciences. Le Journal Canadien Des Sciences Neurologiques·Jinxin HuangXiaofeng Lv
Oct 23, 2018·Stress : the International Journal on the Biology of Stress·Xingxing MoFan Bian
Feb 16, 2017·Oxidative Medicine and Cellular Longevity·Sankarathi BalaiyaKakarla V Chalam
Aug 8, 2021·International Journal of Molecular Sciences·Vadim V KlimontovAnton I Korbut

❮ Previous
Next ❯

Related Concepts

Related Feeds

CV Disorders & Type 2 Diabetes

This feed focuses on the association of cardiovascular diseases in patients with type 2 diabetes.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.