Increase of intracellular calcium is not a cause of pentoxifylline-induced hyperactivated motility or acrosome reaction in human sperm

Fertility and Sterility
A NassarS Oehninger

Abstract

To investigate the effects of the phosphodiesterase inhibitor pentoxifylline on hyperactivated motility and acrosome reaction in human sperm and to determine whether its stimulatory effects occur via increased intracellular calcium levels. Prospective study. Academic tertiary care facility. Healthy male donors. The effects of pentoxifylline on hyperactivated motility, acrosome reaction, and intracellular calcium were studied and compared with the effects of progesterone. Thapsigargin, a known mobilizer of intracellular calcium, also was used as positive control. Hyperactivated motility was assessed by computer-assisted sperm motion analysis using the HTM-IVOS, acrosome reaction was evaluated with the fluorescent probe fluorescein isothiocyanate-labeled Pisum sativum agglutinin, and intracellular calcium was determined by fura-2 using spectrofluorometry. Pentoxifylline significantly increased both hyperactivated motility and acrosome reaction. Enhancement of hyperactivated motility by pentoxifylline in the capacitation medium persisted for up to 5 hours after pentoxifylline was washed from the medium. It also enhanced the percentage of acrosome-reacted spermatozoa after 4 hours of incubation. These effects occurred in the presen...Continue Reading

References

Nov 11, 1990·Cell Calcium·J W Putney
Sep 1, 1990·Journal of Reproduction and Fertility·J M ReesM G Hull
Jun 1, 1985·British Journal of Clinical Pharmacology·C Y HongJ C Fong
Oct 1, 1995·Human Reproduction·H TournayeA Van Steirteghem

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Citations

Nov 18, 2003·Reproductive Biology and Endocrinology : RB&E·Ralf R Henkel, Wolf-Bernhard Schill
Apr 15, 2014·Photochemistry and Photobiology·Shiran ShaharHaim Breitbart
Apr 2, 2015·Reproductive Toxicology·Sandra L GrayWilliam R Boone
Dec 20, 2011·Developmental Biology·Sarit Bar-Sheshet ItachHaim Breitbart
Oct 4, 2016·Journal of Evidence-based Complementary & Alternative Medicine·Sara KhaleghiFarzane Esmaeili
Jan 5, 2002·Radiation Research·Dhruv B PatederRandy N Rosier
Jul 16, 2020·Human Fertility : Journal of the British Fertility Society·Abigail SharpeDavid Miller
Jul 13, 2020·Reproduction in Domestic Animals = Zuchthygiene·Parisa NazariJalal Rostamzadeh

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