PMID: 9525242Apr 3, 1998Paper

Increase of mucous glycoprotein secretion by tumor necrosis factor alpha via a protein kinase C-dependent mechanism in cultured chinchilla middle ear epithelial cells

The Annals of Otology, Rhinology, and Laryngology
J LinS K Juhn

Abstract

Tumor necrosis factor alpha (TNF-alpha), originally defined by its antitumoral activity, is now recognized as a polypeptide mediator of inflammatory and cellular immune response. Recent studies have demonstrated that TNF-(alpha exists in the fluid of otitis media with effusion and, therefore, suggested its possible role in the pathogenesis of mucus hypersecretion. In this study, the effects of TNF-alpha on mucous glycoprotein (MGP) secretion from cultured chinchilla middle ear epithelial cells were examined, and TNF-alpha was found to stimulate MGP secretion in a time- and concentration-dependent manner. The action of TNF-alpha on MGP secretion was significantly and dose-dependently inhibited by TNF-alpha monoclonal antibody; this finding is suggestive of its specificity on MGP secretion. The addition of the protein kinase C inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperidine (H-7) to the culture significantly blocked TNF-alpha-induced MGP secretion, while the calmodulin inhibitor N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7) did not. This suggests that TNF-alpha stimulates MGP secretion via a protein kinase C-dependent mechanism.

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Citations

Sep 9, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Szilárd RezesAnne Pitkäranta
Jun 12, 2012·International Journal of Otolaryngology·Jizhen LinJoseph E Kerschner
Jun 17, 2015·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Beata Zielnik-Jurkiewicz, Wanda Stankiewicz-Szymczak
Aug 24, 1999·The Annals of Otology, Rhinology, and Laryngology·J LinY Kim
Aug 16, 2000·Clinical Otolaryngology and Allied Sciences·H KubbaJ P Birchall

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