Increase of NADPH-diaphorase expression in hypothalamus of stat4 knockout mice.

The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Mee Sook HongJoo-Ho Chung

Abstract

Signal transducer and activator of transcription 4 (STAT4), a STAT family member, mediates interleukin 12 (IL12) signal transduction. IL12 is known to be related to calorie-restricted status. In the central nervous system, IL12 also enhances the production of nitric oxide (NO), which regulates food intake. In this study, the expression of neuronal NO synthase (Nos1), which is also related to food intake, was investigated in the hypothalamic areas of Stat4 knockout (KO) mice using nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) histochemistry, a marker for neurons expressing Nos1 enzyme. Western blots were also performed to evaluate Nos1 and Fos expression. Wild-type Balb/c (WT group, n=10 male) and Stat4 KO mice (Stat4 KO group, n=8 male) were used. The body weight and daily food intake in the WT group were 22.4+/-0.3 and 4.4 g per day, while those in the Stat4 KO group were 18.7+/-0.4 and 1.8 g per day, respectively. Stat4 mice had lower body weight and food intake than Balb/c mice. Optical intensities of NADPH-d-positive neurons in the paraventricular nucleus (PVN) and lateral hypothalamic area (LHA) of the Stat4 KO group were significantly higher than those of the WT group. Western blotting analysis revealed...Continue Reading

References

Jul 1, 1992·Acta Physiologica Scandinavica·S Moncada
Sep 1, 1992·Annals of Neurology·T M DawsonS H Snyder
Jan 1, 1991·Life Sciences·J E Morley, J F Flood
Nov 1, 1983·Journal of Neuroscience Methods·U Scherer-SinglerE G McGeer
May 10, 1996·Neuroscience Letters·J E Morley, M B Mattammal
Oct 31, 1996·Annals of the New York Academy of Sciences·C S ReissZ Bi
Sep 12, 1997·Science·J E Darnell
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D M FruchtJ J O'Shea
Mar 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kavita RamanNicholas W Lukacs

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Citations

Jan 31, 2013·European Journal of Human Genetics : EJHG·Jean-Baptiste Le PichonDouglas C Bittel
Nov 29, 2017·BioMed Research International·Franziska A RadtkeYasir A Syed

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