Increase of P-glycoprotein-mediated drug resistance by hsp 90 beta

Anti-cancer Drugs
J BertramM Kneba

Abstract

The expression of heat shock proteins hsp27, hsp60, hsp70, hsp90 alpha and hsp90 beta in extracts of three cell lines (LoVo DxR, KBChR8-5 and S180 DxR) expressing the MDR (multidrug resistance) positive phenotype as well as in the sensitive parental lines has been investigated. We present evidence that heat shock protein hsp90 beta is associated with the P-glycoprotein (Pgp or P170) one of the most prominent components of the drug resistance machinery. In the doxorubicin-resistant cell line LoVo DxR, but not in the sensitive parental line, hsp90 beta is expressed constitutively as shown by Northern blotting. The expression of hsp90 beta in the sensitive LoVo cell line, however, can be induced by exposure of the doxorubicin-sensitive parental cell line to different stress factors (dexamethasone, doxorubicin, heat treatment or cadmium chloride). We were able to demonstrate that hsp90 beta can be co-precipitated along with Pgp and vice versa. In native agarose gels both proteins migrated together as one single band as shown by Western blot analysis. This intracellular protein-protein interaction may present a mechanism for the modulation of Pgp function possibly by a stabilization of the protein which seems to be attributed to hsp...Continue Reading

Citations

Jun 27, 2000·Journal of Molecular Recognition : JMR·O KirschsteinL Kittler
Aug 10, 2000·Journal of Molecular Recognition : JMR·I Haq, J Ladbury
Jan 15, 1999·Pathology Oncology Research : POR·C Soti, P Csermely
Dec 1, 2009·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Alexander E KabakovVladimir L Gabai
Dec 31, 2003·Bioorganic & Medicinal Chemistry·Jung-Cheng ChangLeung Sheh
Mar 10, 2005·Bioorganic & Medicinal Chemistry·Laurie L ParkerJohn A Hartley
Aug 26, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J BertramM Kneba
Jul 17, 2001·Bioorganic & Medicinal Chemistry Letters·D L BogerM P Hedrick
Feb 5, 2003·Bioorganic & Medicinal Chemistry Letters·Yuqiang WangJames W Larrick
Nov 13, 2003·Bioorganic & Medicinal Chemistry Letters·Yi XiaKuo-Hsiung Lee
Jan 1, 1999·Bioorganic & Medicinal Chemistry Letters·K J AlligoodD D Sternbach
Jan 5, 1999·Bioorganic & Medicinal Chemistry Letters·H H LeeW A Denny
Feb 18, 1999·Bioorganic & Medicinal Chemistry Letters·K HirataK H Lee
Jul 6, 2000·Bioorganic & Medicinal Chemistry·L W DeadyW A Denny
Dec 12, 2001·Bioorganic & Medicinal Chemistry·Julie A SpicerWilliam A Denny
Jan 9, 2003·Bioorganic & Medicinal Chemistry·Ippolito AntoniniSante Martelli
Mar 12, 2003·Bioorganic & Medicinal Chemistry·Yuqiang WangJames W Larrick
Sep 1, 2000·Bioorganic & Medicinal Chemistry·P ValentiM Carrara
Nov 13, 2008·Cytotechnology·M G RumsbyJ R Warr
Apr 2, 2008·Chemical Communications : Chem Comm·Johanna NieselUlrich Schatzschneider
Mar 26, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Wing-Kee LeeFrank Thévenod
Jul 9, 2005·Cancer Cell International·Valeria Ponce de León, Raúl Barrera-Rodríguez
Oct 19, 2007·PLoS Computational Biology·Soundara Raghavan PavithraUtpal Tatu
Dec 24, 2011·Endocrine-related Cancer·Philipp MayerFrank Bergmann
Apr 25, 2006·Biochemical and Biophysical Research Communications·Yuo-Sheng ChangYiu-Kay Lai
Mar 17, 2009·Biochimica Et Biophysica Acta·King Leung Fung, Michael M Gottesman
Apr 9, 2004·FEBS Letters·Amere Subbarao SreedharYu-Fei Shen
Mar 25, 2009·Journal of Cellular Biochemistry·Chih-Wei LoYiu-Kay Lai
Jan 31, 2002·World Journal of Gastroenterology : WJG·Beatrice D Darimont
Apr 3, 2020·Archivum Immunologiae Et Therapiae Experimentalis·Aleksandra Damasiewicz-BodzekKrystyna Tyrpień-Golder
Jan 31, 2002·World Journal of Gastroenterology : WJG·Xian-Ling LiuDai-Ming Fan
Sep 25, 2004·International Journal of Cancer. Journal International Du Cancer·Maria WartenbergHeinrich Sauer
Mar 19, 2008·Molecular Cancer Therapeutics·Agustín HernándezJosé A Pintor-Toro

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