PMID: 9434838Jan 22, 1998Paper

Increase of sensitivity and validity of the SOS/umu-test after replacement of the beta-galactosidase reporter gene with luciferase

Mutation Research
C SchmidM Bachmann

Abstract

The SOS/umu-test with Salmonella typhimurium TA1535/pSK1002 as tester strain is a rapid and valuable bacterial assay for screening of umuC-dependent mutagenic potential of chemical compounds and chemicals relevant to environmental pollution. The initial assay was modified by replacing the beta-galactosidase reporter gene with luciferase. Thereby, the sensitivity of the umu-test was increased significantly and the susceptibility to intensively coloured solutions was reduced. The alternative enzyme assay in the modified umu-test (umu-Luc) represents an independent method which allows to confirm the colorimetric results obtained with the original SOS/umu-test system (umu-Gal) by measuring the beta-galactosidase activity. It has been proved that for several genotoxins an increased induction rate could be achieved by applying the new umu-Luc test.

References

Aug 1, 1992·Molecular Microbiology·R Woodgate, S G Sedgwick
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Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J R de WetM DeLuca
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Citations

Dec 6, 2001·Journal of Microbiological Methods·Magdalena KostrzynskaJack T Trevors
Jun 23, 2005·Biosensors & Bioelectronics·Nobuto MatsuiTomokazu Matsue
Jan 25, 2011·Microbial Biotechnology·Alva BiranShimshon Belkin

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