Increase of xylitol productivity by cell-recycle fermentation of Candida tropicalis using submerged membrane bioreactor

Journal of Bioscience and Bioengineering
Soun-Gyu KwonDeok-Kun Oh

Abstract

Candida tropicalis, an osmophilic strain isolated from honeycomb, produced xylitol at a maximal volumetric productivity of 3.5 g l(-1) h(-1) from an initial xylose concentration of 200 g l(-1). Even at a very high xylose concentration, e.g., 350 g l(-1), this strain produced xylitol at a moderate rate of 2.07 g l(-1) h(-1). In a fed-batch fermentation of xylose and glucose, 260 g l(-1) xylose was added, and the xylitol production was 234 g l(-1) for 48 h, corresponding to a rate of 4.88 g l(-1) h(-1). To increase xylitol productivity, cells were recycled in a submerged membrane bioreactor with suction pressure and air sparging. For each recycle round in cell-recycle fermentation, the average concentration of xylitol produced, fermentation time, volumetric productivity, and product yield were 180 g l(-1), 19.5 h, 8.5 g l(-1) h(-1), and 85%, respectively. When cell-recycle fermentation was started with the cell mass concentrated twofold after batch fermentation and performed for 10 recycle rounds, we achieved a very high productivity of 12 g l(-1) h(-1). The productivity and total amount of xylitol in cell-recycle fermentation were 3.4- and 11.0-fold higher than those in batch fermentation, respectively.

References

Nov 27, 1998·Applied Microbiology and Biotechnology·D K Oh, S Y Kim
Jun 25, 2002·Journal of Industrial Microbiology & Biotechnology·Niklas von WeymarnM Leisola

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Citations

Oct 31, 2013·Journal of Industrial Microbiology & Biotechnology·Guoqiang ZhangYanhe Ma
Dec 3, 2013·Bioresource Technology·Jia ZhangJiong Hong
Mar 9, 2012·Journal of Industrial Microbiology & Biotechnology·Renata BuraSharon L Doty
Jan 12, 2007·Applied Microbiology and Biotechnology·Tom Birger GranströmMatti Leisola
Dec 17, 2009·Applied Microbiology and Biotechnology·Miho SasakiHideaki Yukawa
Apr 29, 2010·Applied Microbiology and Biotechnology·Ke-Ke ChengJin-Ping Li
Sep 2, 2008·Current Opinion in Biotechnology·Olubolaji AkinterinwaPatrick Carmen Cirino
May 5, 2016·Bioresource Technology·Linga Venkateswar RaoSravanthi Koti
May 11, 2011·Letters in Applied Microbiology·Y J JeonP L Rogers
Aug 25, 2018·Applied Microbiology and Biotechnology·Sujit Sadashiv Jagtap, Christopher V Rao
Oct 31, 2017·Frontiers in Microbiology·Diana L Zuza-AlvesGuilherme M Chaves
Dec 12, 2020·Preparative Biochemistry & Biotechnology·Badal C Saha, Gregory J Kennedy

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