Abstract
The interaction of growth factors, such as epidermal growth factor (EGF) with their receptors, on breast cancer cells can lead to the hydrolysis of phospholipids and release of fatty acids, such as arachidonic acid, which can be further metabolized by the lipoxygenase (LO) pathway. Several LO products have been shown to stimulate oncogenes and have mitogenic and chemotactic effects. In this study, we have evaluated the regulation of 12-LO activity and expression in breast cancer cells and tissues. Leukocyte-type 12-LO messenger RNA (mRNA) expression was studied by a specific RT-PCR method in matched, normal, uninvolved and cancer-involved breast tissue RNA samples from six patients. In each of these six patients, the cancer-involved section showed a much higher level of 12-LO mRNA than the corresponding normal section. 12-LO mRNA levels also were greater in two breast cancer cell lines, MCF-7 and COH-BR1, compared with the nontumorigenic breast epithelial cell line, MCF-10F. The growth of the MCF-7 cells was significantly inhibited by two specific LO blockers but not by a cyclooxygenase blocker. Treatment of serum-starved MCF-7 cells with EGF for 4 h led to a dose-dependent increase in the formation of the 12-LO product, 12-hyd...Continue Reading
References
Oct 30, 1992·Biochimica Et Biophysica Acta·S Yamamoto
Sep 1, 1992·Endocrinology·R NatarajanJ Nadler
Nov 1, 1991·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D K BuckmanK L Erickson
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D J ConradE Sigal
Dec 1, 1991·Cell Regulation·B LiuK V Honn
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T IzumiB Samuelsson
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T YoshimotoT Tanabe
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C D FunkG A FitzGerald
Apr 15, 1989·The Biochemical Journal·W L Smith
Sep 1, 1989·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K V HonnJ D Taylor
Oct 16, 1989·Biochemical and Biophysical Research Communications·D P Rose, J M Connolly
Apr 11, 1985·Nucleic Acids Research·J Y TsoR Wu
Jan 1, 1988·Progress in Lipid Research·A A SpectorS A Moore
Dec 15, 1988·Biochemical and Biophysical Research Communications·E SigalJ A Nadel
Feb 1, 1985·Diabetes·J NakaoS Murota
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S L HammondM R Stampfer
Sep 1, 1982·Atherosclerosis·J NakaoS Murota
Jul 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·J A KimJ L Nadler
Dec 1, 1994·Cancer Metastasis Reviews·K V HonnW Hagmann
Jan 1, 1994·Endocrinology·J L GuJ L Nadler
Feb 9, 1993·Biochimica Et Biophysica Acta·A P NaumovG N Mozhayeva
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·R NatarajanJ Nadler
Citations
Oct 20, 2009·Nutrition and Cancer·Heather MernitzXiang-Dong Wang
Jan 12, 2010·Menopause : the Journal of the North American Menopause Society·Pengyuan LiuVolodymyr Dvornyk
Dec 19, 2007·Environmental Health Perspectives·Barry M MarkaverichTrellis Thompson
Dec 7, 2005·Environmental Health Perspectives·Barry M MarkaverichJohn Sharp
Nov 19, 2010·PloS One·Paula M MalobertiErnesto J Podestá
Mar 3, 2012·PloS One·Abhay Kumar SinghSharmistha Dey
Oct 24, 2012·PloS One·Magomed Khaidakov, Jawahar L Mehta
Nov 27, 2002·Medical Hypotheses·Gerhard Spiteller
Oct 31, 2013·Journal of Mammary Gland Biology and Neoplasia·Linda Vona-Davis, David P Rose
May 30, 2013·Familial Cancer·Shuying LiYashuang Zhao
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Tanihiro Yoshimoto, Yoshitaka Takahashi
Jul 18, 2002·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·X Z DingT E Adrian
May 15, 2009·Free Radical Biology & Medicine·Duen-Suey ChouJoen-Rong Sheu
Jun 14, 2005·Biochemical Pharmacology·Khosrow Kashfi, Basil Rigas
Oct 9, 2003·The Journal of Urology·Rikio YoshimuraTatsuya Nakatani