PMID: 6170668Oct 1, 1981Paper

Increased accuracy of absorption cytophotometric DNA values by control of stain intensity

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
D C AllisonT S Johnson

Abstract

A method of improving absorption cytophotometric cellular DNA values by making measurements on Feulgenstained cells at optimal stain absorbances has been developed. Stain intensity can be controlled either by alteration of the Feulgen staining reaction or by selection of "off-peak" wavelengths of light for cytometry. The use of chicken red blood cells as an internal standard, and of a computerized cytometer for the measurements, allows selection of the appropriate off-peak light wavelengths, correction for staining variability at different sites on the same slide, and rapid calculation of cellular DNA values. Cytometry can also be performed at controlled absorbance levels on autoradiographs of 3H-thymidine-labeled cells to allow direct study of the DNA content of nonlabeled G1/G0 and G2/M cells. Use of this technique on mixtures of mouse thymocytes, spleen cells, bone marrow cells, and liver cells gave essentially identical values for G1/G0 cellular DNA content, with coefficients of variation of less than 3%.

Citations

Oct 16, 1999·Cytometry·A E Vinogradov
Jan 14, 2005·Chromosoma·Alexander E Vinogradov
Aug 15, 1984·Biochemical Pharmacology·J GarzónN M Lee
Nov 1, 1985·Journal of Clinical Pathology·F WhiteheadR Whitehead
Jan 1, 1987·Journal of Clinical Pathology·L R JarvisR Whitehead
Nov 14, 2012·Progress in Histochemistry and Cytochemistry·Pasquale ChiecoCornelis J F Van Noorden
Nov 1, 1994·Japanese Journal of Cancer Research : Gann·T YamamotoM Isobe
Sep 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Takahiro IsakaDavid C Allison
May 23, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·David C HardiePaul D N Hebert
Oct 1, 1989·The Journal of Pathology·C D CarterR Whitehead
Dec 1, 1995·Evolution; International Journal of Organic Evolution·Alexander E Vinogradov

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