Increased activity of guanylate cyclase in the atherosclerotic rabbit aorta: role of non-endothelial nitric oxide synthases

British Journal of Pharmacology
A RupinT J Verbeuren

Abstract

1. Experiments were performed to examine the effects of putative non-endothelial nitric oxide on the soluble guanylate cyclase activity of severe atherosclerotic aortae from hypercholesterolaemic rabbits fed a cholesterol rich diet for 45 weeks. 2. The guanosine 3':5'-cyclic monophosphate (cyclic GMP) content of aortae from rabbits fed either a control diet or a diet containing 0.3% cholesterol for 45 weeks was quantified in saline extracts or in trichloracetic acid/either extracts by use of a competitive immunoenzymatic assay. Rabbit anti-cyclic GMP immunoglobulin G was covalently linked to the solid phase, in order to avoid false positive results due to high rabbit immunoglobulin G concentrations in the atherosclerotic saline extracts. 3. Saline extracts of atherosclerotic aortae which were harvested immediately after death (intact aortae) contained about 6 fold more cyclic GMP than control aortae when expressed in pmol cyclic GMP mg-1 protein. The cyclic GMP concentrations in trichloracetic acid/ether extracts of atherosclerotic and control aortae expressed in pmol mg-1 fresh tissue were not significantly different. 4. Neointimal-medial explants from atherosclerotic and control aortae were placed in a physiological saline so...Continue Reading

References

Aug 1, 1992·The Journal of Clinical Investigation·S K Wong, D L Garbers
Nov 27, 1990·European Journal of Pharmacology·T J VerbeurenA G Herman
Dec 1, 1990·The Journal of Clinical Investigation·R L MinorD G Harrison
Dec 1, 1988·Hypertension·R J GryglewskiJ R Vane
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs
Jan 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·S Ylä-HerttualaJ L Witztum
May 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·A J CayatteR A Cohen
Jan 1, 1995·British Journal of Pharmacology·G J SouthanC Thiemermann
May 1, 1993·Journal of Cardiovascular Pharmacology·T J VerbeurenS Simonet
Aug 1, 1993·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·K SchmidtB Mayer
Jun 1, 1994·Circulation·W SteinkeM Hennerici
Jan 1, 1993·Fundamental & Clinical Pharmacology·A Berdeaux
Jun 1, 1993·The Journal of Clinical Investigation·Y OharaD G Harrison

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Citations

Nov 18, 2004·Proceedings of the National Academy of Sciences of the United States of America·Volker O MelicharHarald H H W Schmidt
Jun 5, 2004·European Journal of Pharmacology·Serge SimonetTony J Verbeuren
Sep 13, 2008·American Journal of Hypertension·Delphine Behr-RousselFrançois Giuliano
Feb 25, 2000·Journal of Cardiovascular Pharmacology and Therapeutics·M SchwemmerE Bassenge
Apr 6, 2000·American Journal of Physiology. Endocrinology and Metabolism·A M KahnS Zhang

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