PMID: 7305982Jan 15, 1981Paper

Increased activity of phosphate-dependent glutaminase in liver mitochondria as a result of glucagon treatment of rats

The Biochemical Journal
J LaceyJ D McGivan

Abstract

1. Injection of rats with glucagon leads to an increased effective activity of glutaminase in subsequently isolated liver mitochondria. 2. This effect of glucagon is manifested as a decreased requirement of glutaminase for phosphate in the presence of HCO3-. The HCO3--concentration-dependence is unchanged. 3. The effect of glucagon is lost on disruption of the mitochondria. 4. In accordance with previous reports, incubation of mitochondria in hypo-osmotic media also increases the effective activity of glutaminase. Glucagon increases glutamine hydrolysis at intermediate osmolarities of the suspending medium, but does not affect glutaminase activity when it is already maximally activated by hypo-osmotic conditions. 5. From this and previous work, it seems that hypo-osmotic incubation conditions, EDTA and glucagon may all activate glutaminase by a common mechanism. It is postulated that this mechanism involves modification of the interaction of glutaminase with the mitochondrial inner membrane.

Citations

Nov 13, 2004·Biochimica Et Biophysica Acta·Michael R HaslettM E Brosnan
May 1, 1987·Biological Reviews of the Cambridge Philosophical Society·M D Brand, M P Murphy
Apr 27, 2016·Diabetologia·Russell A Miller, Morris J Birnbaum
Jun 1, 1983·European Journal of Biochemistry·A J VerhoevenA J Meijer
Sep 14, 1999·JPEN. Journal of Parenteral and Enteral Nutrition·B P Bode, W W Souba
Mar 27, 2018·Nature Medicine·Russell A MillerMorris J Birnbaum
Aug 5, 2016·PloS One·Jurandir Fernando ComarAdelar Bracht
Apr 15, 1985·European Journal of Biochemistry·J D McGivanN M Bradford
Jul 15, 1987·European Journal of Biochemistry·C LenzenD Häussinger
Dec 1, 1991·European Journal of Clinical Investigation·H HeindorffHendrik Vilstrup
Dec 12, 2007·The Journal of Biological Chemistry·Lise MadsenKarsten Kristiansen
Oct 31, 2009·World Journal of Hepatology·Verena Puxbaum, Lukas Mach
May 24, 2011·World Journal of Clinical Oncology·Vaclav VetvickaJana Vetvickova
Mar 17, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Katrine D GalsgaardJens J Holst
Jan 1, 1995·Advances in Enzyme Regulation·J T BrosnanS A Squires
Jul 19, 2002·Advances in Enzyme Regulation·J T BrosnanItzhak Nissim
Dec 15, 1992·Biochimica Et Biophysica Acta·N C VincentG Baverel
Dec 12, 1991·Biochimica Et Biophysica Acta·D Häussinger, F Lang

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.