Increased Alternative Splicing as a Host Response to Edwardsiella ictaluri Infection in Catfish

Marine Biotechnology
Suxu TanZhanjiang Liu

Abstract

Alternative splicing is the process of generating multiple transcripts from a single pre-mRNA used by eukaryotes to regulate gene expression and increase proteomic complexity. Although alternative splicing profiles have been well studied in mammalian species, they have not been well studied in aquatic species, especially after biotic stresses. In the present study, genomic information and RNA-Seq datasets were utilized to characterize alternative splicing profiles and their induced changes after bacterial infection with Edwardsiella ictaluri in channel catfish (Ictalurus punctatus). A total of 27,476 alternative splicing events, derived from 9694 genes, were identified in channel catfish. Exon skipping was the most abundant while mutually exclusive exon was the least abundant type of alternative splicing. Alternative splicing was greatly induced by E. ictaluri infection with 21.9% increase in alternative splicing events. Interestingly, genes involved in RNA binding and RNA splicing themselves were significantly enriched in differentially alternatively spliced genes after infection. Sequence analyses of splice variants of a representative alternatively spliced gene, splicing factor srsf2, revealed that certain spliced transcript...Continue Reading

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Citations

Jan 30, 2019·The Journal of Experimental Biology·Timothy M Healy, Patricia M Schulte
Dec 16, 2020·Epigenetics : Official Journal of the DNA Methylation Society·Francisco LeivaRodrigo Vidal
Nov 28, 2018·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Suxu TanZhanjiang Liu
Apr 6, 2019·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Suxu TanZhanjiang Liu

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Methods Mentioned

BETA
RNA-Seq
electrophoresis
PCR

Software Mentioned

Cufflinks
BLAST
FastQC
PANTHER
Primer3
CFX Manager
rMATS
FASTX
TopHat2
ASTALAVISTA

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Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.