Increased amounts of the Aspergillus metabolite D-mannitol in tissue and serum of rats with experimental aspergillosis

The Journal of Infectious Diseases
B WongK Jayasimhulu

Abstract

Several Aspergillus species produce large amounts of the hexitol D-mannitol in vitro, but it is not known whether these species also produce D-mannitol in vivo. Serum samples and homogenized tissues were analyzed from rats pretreated with cortisone and cyclophosphamide and then given 2 x 10(6) preincubated conidia of Aspergillus fumigatus intravenously. The resulting infection was lethal by 48 h and was characterized by much more severe disease in the liver than in the kidneys, spleen, or lungs. A compound present in increased amounts in the livers and sera of the infected rats was shown to be D-mannitol by gas chromatography (GC) and GC/mass spectrometry and enzymatically. Quantitative analysis by GC showed that the infected rats had more D-mannitol in their livers (but not in their lungs or kidneys) after 12 h (P less than .01 at 12, 24, and 36 h) and higher serum D-mannitol concentrations and serum D-mannitol/creatinine ratios after 36 h (P less than .05) than did uninfected controls. These results indicate that A. fumigatus can produce and release sufficient D-mannitol in the tissues of infected animals to raise serum D-mannitol levels. Thus, D-mannitol is a potential diagnostic marker for aspergillosis.

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Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.