PMID: 6975941Oct 1, 1981Paper

Increased ascorbic acid content in chronic lymphocytic leukemia B lymphocytes

Proceedings of the National Academy of Sciences of the United States of America
L F LiebesR Silber

Abstract

Human lymphocyte extracts analyzed by high-performance liquid chromatography reveal a major UV-absorbing peak that was shown to be ascorbic acid by spectral, chemical, and enzymatic criteria. Because this peak appeared very prominent in the elution profile of chronic lymphocytic leukemia (CLL) lymphocyte extracts, we measured the ascorbic acid content in lymphocytes from the blood of normal subjects and untreated patients with chronic lymphocytic leukemia. A significantly higher concentration of 111 +/- 15.3 nmol per 10(8) cells (mean +/- SEM) was found in CLL lymphocytes than in normal blood lymphocytes, which contained 42.2 +/- 3.3 nmol per 10(8) cells. Selective enrichment with B and T cells showed that this difference was limited to the chronic lymphocytic leukemia B cell, which had a 5- to 15-fold higher content of ascorbic acid than normal B cells had. In contrast, the ascorbic acid level was similar in normal and CLL T cells. The very high ascorbic acid content provides the chronic lymphocytic leukemia B cell with a reducing substance that could react with oxidants or free radicals.

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Citations

Oct 13, 2006·Apoptosis : an International Journal on Programmed Cell Death·Isabel Perez-CruzDavid W Golde
Mar 29, 1985·Biochimica Et Biophysica Acta·R L StahlR Silber
Jun 1, 1983·Analytical Biochemistry·R L StahlR Silber
Jun 12, 2008·The Journal of Toxicological Sciences·Masayoshi TakabatakeKunitoshi Mitsumori
Dec 13, 2003·Free Radical Research·Victor M VictorMonica De la Fuente
Jun 1, 1982·Hospital Practice·R Silber

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