Increased atypical PKC expression and activity in the phrenic motor nucleus following cervical spinal injury.

Experimental Neurology
C H GuentherGordon S Mitchell

Abstract

Atypical protein kinase C (aPKC) isoforms are expressed in phrenic motor neurons, a group of motor neurons critical for breathing. Following C2 cervical hemisection (C2HS), spontaneous plasticity occurs in crossed-spinal synaptic pathways to phrenic motor neurons, at least partially restoring inspiratory phrenic activity below the injury. Since aPKCs are necessary for synaptic plasticity in other systems, we tested the hypothesis that C2HS increases aPKC expression and activity in spinal regions associated with the phrenic motor nucleus. C2 laminectomy (sham) or C2HS was performed on adult, male Lewis rats. Ventral spinal segments C3-5 were harvested 1, 3 or 28 days post-surgery, and prepared for aPKC enzyme activity assays and immunoblots. Ventral cervical aPKC activity was elevated 1 and 28, but not 3, days post-C2HS (1 day: 63% vs sham ipsilateral to injury; p<0.05; 28 day: 426% vs sham; p<0.05; no difference in ipsilateral vs contralateral response). Total PKCζ/ι protein expression was unchanged by C2HS, but total and phosphorylated PKMζ (constitutively active PKCζ isoform) increased ipsilateral to injury 28 days post-C2HS (p<0.05). Ipsilateral aPKC activity and expression were strongly correlated (r(2)=0.675, p<0.001). In ...Continue Reading

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Citations

Nov 16, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kristi A StreyTracy L Baker-Herman
Jul 24, 2013·The Journal of Physiology·Oleg BroytmanTracy L Baker-Herman
Apr 10, 2020·Neuronal Signaling·Sophie M HapakSourav Ghosh

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