Abstract
The brain uptake and brain to blood efflux transport of (14)C-GABA were studied in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats using 20 min bilateral in situ brain perfusion in rats anesthetized using urethane. The volume of distribution (Vd) of (14)C-GABA into cerebrospinal fluid (CSF) and brain regions (cortex, diencephalon, cerebellum, and brain stem) was significantly greater in SHR than in the corresponding regions in WKY rats (p<0.05). The estimated Vd value of (14)C-GABA in CSF of SHR was 3.4 fold greater than that in WKY. Also compared to WKY, the Vd of (14)C-GABA into cerebellum and cortex of SHR was 15.3 fold and 19.4 fold greater, respectively. Although the study of blood-brain barrier (BBB) integrity using (3)H-mannitol revealed increased paracellular permeability at the brain capillaries of SHR when compared to WKY rats, this was found to be only partially responsible for the increased (14)C-GABA uptake. The study of brain to blood efflux transport of (14)C-GABA (after loading of brain with (14)C-GABA by vascular perfusion) revealed that the half-time of elimination was significantly shorter in SHR (5.35+/-0.66 min) than in WKY rats (14.83+/-1.94 min), (p<0.001). HPLC analysis rev...Continue Reading
References
Jul 1, 1979·Journal of Neurochemistry·J W FerkanyS J Enna
May 1, 1991·Annals of Neurology·P H ChanC J Epstein
Jan 1, 1988·Acta Neuropathologica·K FredrikssonB B Johansson
Oct 1, 1986·Brain Research·J LermaR Martín del Río
Jan 1, 1985·Acta Neuropathologica·K FredrikssonB B Johansson
Mar 1, 1983·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·R G BlasbergC S Patlak
Feb 1, 1980·Neuropharmacology·H H Frey, W Löscher
Jun 27, 1995·Brain Research. Developmental Brain Research·J E PrestonM B Segal
Jan 1, 1993·Life Sciences·J P TangS Melethil
Nov 24, 1993·Journal of Chromatography·P J BorgD A Taylor
Dec 21, 1995·Brain Research. Developmental Brain Research·H al-SarrafM B Segal
Nov 1, 1996·Journal of Neurosurgery·S MikawaP H Chan
May 1, 1997·Stroke; a Journal of Cerebral Circulation·S Nag, D W Kilty
Aug 18, 1997·Brain Research. Developmental Brain Research·H al-SarrafM B Segal
May 11, 1999·Brain Research. Brain Research Protocols·G PinnaA Baumgartner
Jan 27, 2000·Respiration Physiology·B HoopH Kazemi
Sep 29, 2000·Neuroscience·M SabbatiniF Amenta
Feb 14, 2002·Mechanisms of Ageing and Development·Maurizio SabbatiniRoberto Avola
Dec 14, 2002·Brain Research. Developmental Brain Research·Hameed Al-Sarraf
May 24, 2003·Brain Research·Hameed Al-Sarraf, Laila Philip
Oct 17, 2003·Life Sciences·M KayaF Korkut
Mar 26, 2004·Acta Neuropathologica·Masaki UenoKenji Kanenishi
Citations
Oct 3, 2013·Journal of Cardiovascular Pharmacology·Shuhei KobuchiYasuo Matsumura
Jan 1, 2010·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·K MurphyR H Ettinger
Sep 22, 2009·European Journal of Pharmacology·Shuhei KobuchiYasuo Matsumura
Dec 9, 2014·Clinical and Experimental Pharmacology & Physiology·Shuhei KobuchiKazuhide Ayajiki
May 1, 2012·Life Sciences·Rie SuzukiYasuo Matsumura
Jun 3, 2011·The Journal of Pharmacology and Experimental Therapeutics·Shuhei KobuchiYasuo Matsumura
Aug 28, 2021·Nutrients·Hellen A Oketch-RabahJoseph M Betz