Increased cardiac angiotensin II receptors in angiotensinogen-deficient mice

Hypertension
Y SumidaM Ishii

Abstract

Two subtypes of angiotensin II (Ang II) receptors, type 1 (AT1-R) and type 2 (AT2-R), have been identified in the heart. However, little is known about the regulation of cardiac AT1-R and AT2-R by Ang II in vivo. Thus, we examined cardiac AT1-R and AT2-R in angiotensinogen-deficient (Atg-/-) mice that are hypotensive and lack circulating Ang II. Cardiac Ang II receptors (Ang II-R) were assessed by radioligand binding with 125I-[Sar1,Ile8]-Ang II in plasma membrane fractions. AT1-R and AT2-R were distinguished using their specific antagonists CV-11974 and PD123319, respectively. Total densities of Ang II-R and AT1-R density were significantly greater in the Atg-/- mice than Atg+/+ mice (31.1+/-2.8 versus 18.8+/-2.1, 28.7+/-3.0 versus 16.9+/-2.3 fmol/mg protein, P<.01, respectively), and AT2-R showed a slight but not significant increase in Atg-/- mice relative to Atg+/+ control animals. Kd values were not different between the two groups. In contrast to binding experiments, levels of Ang II type 1a receptor (AT1a-R) and AT2-R mRNA did not differ between Atg-/- and Atg+/+ mice. These results suggest that lack of Ang II may upregulate AT1-R through translational and/or posttranslational mechanisms in Atg-/- mice.

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Citations

Mar 13, 2009·Current Hypertension Reports·Rajesh KumarKenneth M Baker
Feb 19, 2016·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Hong LuAlan Daugherty
Mar 11, 2016·Endocrine Journal·Junichiro SatoTaroh Iiri
Mar 22, 2007·Respiratory Physiology & Neurobiology·Harold D Schultz, Yu Long Li
Mar 11, 2004·American Journal of Physiology. Renal Physiology·Alex PaliegeTianxin Yang
Aug 24, 2006·Physiological Genomics·Gustavo J J SilvaJose E Krieger

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